Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,254,4800AC57.1% 4.4 / 13.2 14V137G (GTG→GGG) fruAPTS fructose transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base A (5/1);  new base C (0/8);  total (5/9)
Fisher's exact test for biased strand distribution p-value = 3.00e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACGCCAGTC  >  NZ_CP009273/2254410‑2254551
                                                                      |                                                                       
aTCCGCTGCTGCGACTTCTTCGGGAGAGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGtttttt                                                      >  2:335165/1‑90 (MQ=255)
    gctgctGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCt                                                  >  1:38664/1‑90 (MQ=255)
     ctgctgCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCtt                                                 <  1:90932/90‑1 (MQ=255)
         tgcGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCTTCGGt                                             >  2:284251/1‑90 (MQ=255)
                         gTGATTGCTTTCCCCGCGCCAACAGAACCACGGGTTTCAACTTTCCCCCCCCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgcc                             <  1:184474/90‑1 (MQ=255)
                         gTGATTGCATTCCCCGCGCCAACAGAACCACGGTTTTCAACTTTCCCCCCCCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgcc                             <  1:284251/90‑1 (MQ=255)
                         gTGATTGCATTCCCCGCGCCAACAGAACCACGGGTTTCAACTTCCCCCCCCCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgcc                             <  2:69218/90‑1 (MQ=255)
                           gtttGCTTTCCCCCCCCCAACAAACCCCCGGTTTTAAACTTCCCCCCCCCAGCCCCGTTTTTTCGCTTCGGTTTCAATGGCTTCAgccgc                           <  2:94590/88‑1 (MQ=255)
                           gATTGCATTCCCCGCGCCAACAGAACCCGGGTTTTCAATTTCCCCCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgccgc                           <  2:346386/90‑1 (MQ=255)
                           gATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgccgc                           >  1:159377/1‑90 (MQ=255)
                                     cccGCGCCAACAGACCCCCGGTTTTAAACTTTCCCCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGgtg                 <  1:330158/90‑1 (MQ=255)
                                      ccccgcCAACAGACCCGCGGTTTTCAACTTCCCCCCCCCACCCCCTTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGgtgt                <  2:324820/87‑1 (MQ=255)
                                         cgcCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGtgtgtg             >  1:324820/1‑90 (MQ=255)
                                                    ccACGGGTTTCAACTTTCCCCCCCCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACGCCAGTc  <  1:340139/90‑1 (MQ=255)
                                                                      |                                                                       
ATCCGCTGCTGCGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACGCCAGTC  >  NZ_CP009273/2254410‑2254551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑