| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,175,946 | 0 | C | G | 53.8% | 2.0 / 10.0 | 13 | A13G (GCA→GGA) | zupT | zinc transporter ZupT |
| Reads supporting (aligned to +/- strand): ref base C (3/3); new base G (7/0); total (10/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGG > NZ_CP009273/3175857‑3176034 | atcaGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGc < 1:39576/90‑1 (MQ=255) gTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGcc > 2:135985/1‑90 (MQ=255) gAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGc < 1:319733/60‑1 (MQ=255) tttATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCCCGGTTTTTTGGGCGGTTTTCCGCGTTCTCg > 2:155750/1‑90 (MQ=255) gAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCCCCGTTTTTGGGGCGTTTTTTGGCGTTCTCCGGCgaaaa > 2:152799/1‑90 (MQ=255) gAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCCCGTTTTTTGGGGCGTTTTTCGGCGGTCTCGGGGaaaaa > 1:62114/1‑90 (MQ=255) tGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTTTTGGCGCGTTTTTCGGCGTTCTCGGGCAAAAAaccc > 1:24849/1‑89 (MQ=255) tGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCCCGGTTTTTTGGGCGGTTCTCTGCGTTCTCGGGCGAAAAACCCCCAAcc > 2:144918/1‑90 (MQ=255) tctcATTCTGACCATACTGGCGGGGGGAGCCCCGCTTTTTTGGGCGCTTTTCCGCGGTCTCGGGCAAAAACCCCCCAACCCCTTTCtagg > 1:286067/1‑87 (MQ=255) tctcATTCTGACCATACTGGCGGGGGGAGCCACGTTTTTTTGCGCGTTTTTCGGCGTTCTCGGGCCAAAAACCCCGGACCCCTTACttgg > 2:11953/1‑87 (MQ=255) ttCTGACCATACTGGCGGGGGCAGCCACGTTTTTTTGCGCGGTTTTTCGCGTTCTCGGGCAAAAAACCCCCAACCCCCTACTTGCGtttt > 2:22671/1‑90 (MQ=255) tACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGtt > 1:145760/1‑90 (MQ=255) ggggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 1:63552/90‑1 (MQ=255) ggggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 2:286067/90‑1 (MQ=255) ggggAGCCACGTTTTTTTGGGGGGTTTTCGGCGGTTTCGGGGGAAAAACCTCGGACCGCTTACTGGCGGTTTTTCTTGGGTTTGcggggg > 2:322177/1‑90 (MQ=255) gCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCgggg < 1:68349/90‑1 (MQ=255) | ATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGG > NZ_CP009273/3175857‑3176034 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |