| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,342,890 | 0 | G | C | 53.8% | 6.3 / 13.3 | 13 | G93G (GGC→GGG) | mtgA | monofunctional biosynthetic peptidoglycan transglycosylase |
| Reads supporting (aligned to +/- strand): ref base G (4/2); new base C (0/7); total (4/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCTTTCTCAATGGAAGCGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACC > NZ_CP009273/3342801‑3342971 | gtctgtTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCTTTCTCAATGGAAGCGACATCAAAg > 2:193411/1‑90 (MQ=255) cGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCTTTCTCAATGGAAGCGACATCAAAGCCCCAGTGCTCAgg > 1:151065/1‑90 (MQ=255) cgcTCGTTGTGCGCCAGGGCTTTCTCAATGGAAGCGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGcc < 1:193411/90‑1 (MQ=255) cgcTCGTTGTGCGCCAGGGCTTTCTCAATGGAAGCGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGcc > 2:281182/1‑90 (MQ=255) ctctTTGTGCGCCGGGGTTTTCTCAAGGGAAGCGACATCAAACCCCCAGTGCTCAGGAAATTTCTGATCTTCGGCGGCAATCACCGCCAg < 2:95548/90‑1 (MQ=255) cTCGTTGTGCCCCGGGGTTTTCTAAAGGGAAGCGAAATCAAACCCCCAGTGCTCAGGAAATTTCTGATTTTCGGCGGCAATCACCGCCAg < 2:63573/90‑1 (MQ=255) cAGGGCTTTCTCAATGGAAGCGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACgg > 2:224427/1‑90 (MQ=255) ggtcTTTCTCAATGGAAGCGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCg < 1:313670/87‑1 (MQ=255) gggCTTTCTCAATGGAAGCGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCg > 1:66633/1‑90 (MQ=255) ttCTCAAGGGAAGCGACATCAAACCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGAtt < 1:346889/90‑1 (MQ=255) ttCTCAAGGGAAGCGACATCAAACCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGAtt < 2:70976/90‑1 (MQ=255) aaTGGAAGCGACATCAAACCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATc < 1:204208/90‑1 (MQ=255) gACATCAAACCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAACCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGAc < 1:310336/90‑1 (MQ=255) aCATAAAACCCCCAGTGCTCAGGAATTTTCTGTTCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGAcc < 1:367306/90‑1 (MQ=255) | GTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCTTTCTCAATGGAAGCGACATCAAAGCCCCAGTGCTCAGGAAATTTCTGATCTTCTGCGGCAATCACCGCCAGTCCCATCCACGGCGAGATTTGATCCATACTGACC > NZ_CP009273/3342801‑3342971 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |