Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,357,7550AT57.1% ‑1.9 / 12.3 14P530P (CCA→CCT) yhcDfimbrial biogenesis outer membrane usher protein
Reads supporting (aligned to +/- strand):  ref base A (4/2);  new base T (0/8);  total (4/10)
Fisher's exact test for biased strand distribution p-value = 1.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ACTGGCAGCATCAACTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATG  >  NZ_CP009273/3357679‑3357830
                                                                            |                                                                           
aCTGGCAGCATCAACTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCaa                                                                >  2:247751/1‑90 (MQ=255)
  tGGCAGCATCATTTTAGTGCTTCAAAAAAAAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCTTTTGGCAGATCAAac                                                              <  2:37939/90‑1 (MQ=255)
           cAACTTAGTGCTTCAAAAAAAAATAAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCcaca                                                     <  1:304222/90‑1 (MQ=255)
            gatttAGTGCTTAAAAAAATAATGAAGACGAGGGTGTTTTGTTCTACGTCAACATCCGTATTCCTTTTGGCAGATCAAACACCGCCacac                                                    <  2:281293/87‑1 (MQ=255)
            aaCTTAGTGCTTAAAAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCTTTTGGCAGATCAAACACCGCCacac                                                    <  1:331329/90‑1 (MQ=255)
                   tgtttCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCTTTTGGCAGATCAAACACCGCCACACTGTATAc                                             <  1:116468/87‑1 (MQ=255)
                       tCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGc                                         >  1:281293/1‑90 (MQ=255)
                                       aCGATGGTGTTCTGTTCTACGTAAACACCATTTTTCCTTTTGGCAGACCAAACCCCGCCACACTGTATACTCGCCATGACGATCATaaaa                         <  2:347944/90‑1 (MQ=255)
                                          aTGGTGATCTGTTCTACGTCAACATCAGTATTCCTTTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAAccc                      <  1:23366/90‑1 (MQ=255)
                                               gATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTAt                 >  1:240901/1‑90 (MQ=255)
                                               gATCTGTTCTACGCAAACATCAGTTTTCCTTTTGGAAGATAAAACCCCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTAt                 <  1:215849/90‑1 (MQ=255)
                                                        tACGTAAACATCAGTATTCCTTTTGGAAAATAAAACCCCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGgt        <  2:93173/90‑1 (MQ=255)
                                                         aCGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGgtg       <  1:113906/90‑1 (MQ=255)
                                                              aaCATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATg  >  2:110801/1‑90 (MQ=255)
                                                                            |                                                                           
ACTGGCAGCATCAACTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATG  >  NZ_CP009273/3357679‑3357830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑