| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,780 | C→T | intergenic (+149/‑43) | kefC → / → folA | glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,780 | 0 | C | T | 92.9% | 43.4 / ‑3.7 | 14 | intergenic (+149/‑43) | kefC/folA | glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (7/6); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.95e-01 | |||||||||||
GAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGT > NZ_CP009273/49695‑49851 | gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGTTTTa < 1:356434/90‑1 (MQ=255) gTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGTTTTACGTATAGTGGCGACAAt > 1:266006/1‑90 (MQ=255) gTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGTTTTACGTATAGTGGCGACAAt > 2:46885/1‑90 (MQ=255) gAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGTTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaa > 1:139541/1‑90 (MQ=255) atatAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGTTTTa < 1:22299/56‑1 (MQ=255) atatAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGTTTTa > 2:22299/1‑56 (MQ=255) ttCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGTTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc > 1:89269/1‑90 (MQ=255) ttCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGTTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc > 1:93814/1‑90 (MQ=255) ttCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGTTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc < 2:143163/90‑1 (MQ=255) atcCTCGCACCAGTCGACGACGGTTTACGTTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcg > 1:226471/1‑90 (MQ=255) atcCTCGCACCAGTCGACGACGGTTTACGTTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcg < 2:287849/90‑1 (MQ=255) cGCACCAGTCGACGACGGTTTACGTTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt < 2:188985/90‑1 (MQ=255) ccAGTCGACGACGGTTTACGTTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCg < 2:293758/90‑1 (MQ=255) aGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGt > 2:203748/1‑90 (MQ=255) | GAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGT > NZ_CP009273/49695‑49851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |