Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,511,988	C→A	E146* (GAG→TAG)	aroK ←	shikimate kinase AroK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,511,988	0	C	A	100.0%	23.6 / NA	8	E146* (GAG→TAG)	aroK	shikimate kinase AroK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (4/4);  total (4/4)

GCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGGTGTTTCAACG  >  NZ_CP009273/3511908‑3512052                                                                                 |                                                                 gCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTATTCATACAg                                                         <  2:319280/90‑1 (MQ=255)                                            cTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTATTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACgcggc              >  2:232696/1‑90 (MQ=255)                                              ttGATCATCAGTACGAATGGTCACGTCGGCAATCTATTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGcggcgg            <  1:377067/90‑1 (MQ=255)                                                        gTACGAATGGTCACGTCGGCAATCTATTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGGTGTTTCAACg  >  2:270706/1‑90 (MQ=255)                                                          aCGAATGGTCACGTCGGCAATCTATTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCa                    >  1:14036/1‑70 (MQ=255)                                                          aCGAATGGTCACGTCGGCAATCTATTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCa                    <  2:14036/70‑1 (MQ=255)                                                                  tCACGTCGGCAATCTATTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCAcgc                 <  1:381724/65‑1 (MQ=255)                                                                  tCACGTCGGCAATCTATTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCAcgc                 >  2:381724/1‑65 (MQ=255)                                                                                 |                                                                 GCTTTCCAGCATGTGAATAATCTGGTTTGCAACCACTTTAGCGCTTTGATCATCAGTACGAATGGTCACGTCGGCAATCTCTTCATACAGCGGATTGCGTTCATTGGCCAACGCTTCCAGAACTTCACGCGGCGGTGTTTCAACG  >  NZ_CP009273/3511908‑3512052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑