| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,806,632 | 0 | C | T | 40.5% | 29.0 / 15.4 | 37 | S181L (TCG→TTG) | coaBC | bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate‑‑cysteine ligase CoaBC |
| Reads supporting (aligned to +/- strand): ref base C (2/20); new base T (15/0); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.45e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTA > NZ_CP009273/3806549‑3806718 | tGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGGGCCTTTTTTGcccct > 1:192171/1‑88 (MQ=255) aGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAAc < 1:182822/90‑1 (MQ=255) aGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAAc < 1:355040/90‑1 (MQ=255) ttGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGaa < 2:256999/90‑1 (MQ=255) ttGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGaa < 1:478957/90‑1 (MQ=255) ttGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGaa < 1:422131/90‑1 (MQ=255) gATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGGAGGGCATTTTTTTCCCGGCCACGACCCGAAAAATCTg > 2:29437/1‑90 (MQ=255) atatCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTTAACGACCTGAAACATCTGa < 1:491964/90‑1 (MQ=255) gTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCCTTTTTTGCCCGGCAACGACCTGAAACATCTGAACAttt > 1:271712/1‑89 (MQ=255) gggCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTTGCCCGTCAAAGACCTGAAAAATCTGAACATTATGAtt > 1:34425/1‑90 (MQ=255) aaTGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTTGCCCGTCAACGACCTGAAACATCTGAACATTATGATTAccgc > 1:24244/1‑90 (MQ=255) aaTGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTTGCCCGTCAACGACCTGAAACATCTGAACATTATGATTAccgc > 1:108926/1‑90 (MQ=255) cGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTTGCCCCTCAACGACCCGAAAAAACTGAACATTATGATTACCGCCGGccc > 2:245383/1‑90 (MQ=255) gATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCg < 1:516906/90‑1 (MQ=255) gATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTCCGCCCGTCACCGACCTGAAACATTTGAACATTATGATTACCGCCGGCCCg < 2:420552/90‑1 (MQ=255) ttGTGGATATGGCGGTAGCGCATTTTTTGCCCGTCAACGACCTGAAAAATCTGAACATTATGATTACCGCCGGCCCGACGCGGGAACCGc > 1:233486/1‑90 (MQ=255) tgtgGATATGGCGGTAGCGCATTTTTTTCCCCTCCACGACCCGAAACATCTGAACATTATGATTACCCCCCGCCCCACGCGTGGACCGcc > 2:205889/1‑89 (MQ=255) gtgGATATGGCGGTAGCGCATTTTTTTCCCGGCAACCACCCGAAAAAACTGAAAATTATGATTTCCGCCGGCCCCACGCGGGAAcccccc > 2:486545/1‑86 (MQ=255) gtgGATATGGCGGGAGCGCATTTTTCACCCCTCAACGACCCAAAAAAACTAAACATTATTATTAACCCCCGCCCGCCCCGTGAAACGccc > 1:509320/1‑88 (MQ=255) gATATGGCGGTAGCGCATTTTTTCCCCGTCTACGACCCGAAAAATCTGAACATTATGATTACCCCCGGCCCGACGCGGGGAACGCTCGAt > 2:249554/1‑90 (MQ=255) tatGGCGGTAGCGCATTTTTTGCCCGTCAACGACCTGAAAAATCTTAACATTATGATTACCGCCGGCCCGACGCGGGAACCGCCCGATcc > 1:376804/1‑90 (MQ=255) tatGGCGGTAGCGCATTTTTCGCCCGTCAAGGCCCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATcc < 2:274072/90‑1 (MQ=255) tatGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATcc < 2:221094/90‑1 (MQ=255) tGGCGGTAGCGCATTTTTTGCCCGTCAACGACCTGAAACATCTGAACATTATTATTACCGCCGGCCCGACGCGTGAACCCCTCGATCCgg > 1:407501/1‑90 (MQ=255) tGGCGGTAGCGCATTTTTTGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCCACGCGTGAACCGCCCGATcccg > 1:271322/1‑88 (MQ=255) tGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCgg < 1:359443/90‑1 (MQ=255) gCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTg < 1:486545/90‑1 (MQ=255) cGGTAGCGCATTTTTTGCCCGTCAACGACCTGAAACATCTGAAAATTATGATTACCCCCGGCCCCACGCGTGAACCGCTCGATCCGGTgg > 2:359443/1‑89 (MQ=255) cGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGc < 1:534193/90‑1 (MQ=255) cGGTAGCGCATTTTTCGCCCGCCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGc < 2:617795/90‑1 (MQ=255) gTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGt < 1:417984/90‑1 (MQ=255) gcgcATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTa < 2:429000/43‑1 (MQ=255) gcgcATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTa > 1:429000/1‑43 (MQ=255) gcgcATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTtat < 1:3435/90‑1 (MQ=255) gcATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTatat < 2:24244/90‑1 (MQ=255) tttttCGCCCGTCAACGCCCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATAtctc < 2:108926/90‑1 (MQ=255) tttCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTa < 1:77073/90‑1 (MQ=255) | TGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTA > NZ_CP009273/3806549‑3806718 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |