| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,058,958 | 0 | G | A | 60.9% | 0.5 / 20.6 | 23 | L57L (CTG→TTG) | yihO | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base G (6/3); new base A (0/14); total (6/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.32e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.74e-05 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAA‑CTT‑CGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058870‑4059038 | aaTTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAA‑CTT‑CGCCACCAGa < 1:237481/90‑1 (MQ=255) aTTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAA‑CTT‑CGCCACCAGaa < 1:484716/90‑1 (MQ=255) tGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAA‑CTT‑CGCCACCAGAAAGa > 2:132772/1‑90 (MQ=255) ttAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAA‑CTT‑CGCCACCAGAAAGATAATc > 2:180050/1‑90 (MQ=255) ttAGCCCAAATATTTTTCCGCGAGTCGAAAAAAAACCGGGGGAGAATATCGGAAAACGGGAAAAAAAC‑CTC‑CCCCACAAAAAAAATaacc < 2:430196/90‑3 (MQ=255) cccaaaTATTTTCCCGGGAGACGAAAAAAAACCGGGTGAGCATATGGGAAAACGGGGAAAAAAA‑CTT‑CGCCACAAAAAAAATAACCCCGc < 2:597580/86‑1 (MQ=255) tttCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAA‑CTT‑CGCCACCAGAAAGATAATCCCGCCATAGTAAGc > 1:248557/1‑90 (MQ=255) tttCCGCGAGTCGAAAAAAAACCCGGGGAGCATATGGGAAAACGCGATAAAAAC‑CTT‑CCCCCCCAAAAAGATAACCCCGCCATAGTAAGc < 1:520829/90‑1 (MQ=255) ttcccGGGAGTCGAAAAAAAACCGGGGGAGCATATGGGAAAACGCGAAAAAAAC‑CTC‑CCCCACAAAAAAGAAAACCCCGCCATAGTAAGc < 2:501262/87‑1 (MQ=255) gcgAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAA‑CTT‑CGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCa > 2:403482/1‑90 (MQ=255) gcgAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAA‑CTT‑CCCCACCAAAAAAATAATCCCGCCATAGTAAGCAGGCa < 1:155586/90‑1 (MQ=39) aaaCCCGGTGAGCATATGGGTAACCGGGGAAAAAAA‑CTT‑CCCCACCAAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATc < 1:295363/90‑1 (MQ=255) ggTGAGCATATGGGAAAACGCGGAAAAAAA‑CTC‑CCCCCCCAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtata < 1:180050/90‑1 (MQ=255) cATATCGGAAAACGCGGAAAAAAA‑CTT‑CCCCCCCAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAAttt < 1:135516/90‑1 (MQ=255) aatatCGGAAAACGCGAAAAAAAA‑CTT‑CGCCCCAAAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAAttt < 1:379904/89‑1 (MQ=255) ccGGAAAGCGCGGAAAAAAC‑CTC‑CGCCACAAGAAAAATACCCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagg < 1:69487/89‑1 (MQ=255) cggaaaCCCGGGAAAAAACCTC‑CCCCACAAAAAAAAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagga < 2:340549/87‑1 (MQ=255) gaaaaCGGGGAAAAAAC‑CTC‑CGCCCCAAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGgaga < 2:507494/88‑1 (MQ=255) cgcgGTAAAAAA‑CTT‑CGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACag > 2:133116/1‑90 (MQ=255) ggggaaaaaa‑CTTCCCCCCCCAAAAAAAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACaga < 1:526963/86‑1 (MQ=255) aaaaac‑CTC‑CCCCCCCAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTAcc < 1:139192/90‑1 (MQ=255) ccTT‑CCCCACAAAAAAGAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGc < 1:308613/89‑1 (MQ=255) t‑CGCCACCAGAGAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCaga > 1:365667/1‑90 (MQ=255) | AATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAA‑CTT‑CGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058870‑4059038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |