| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,101,675 | 0 | G | T | 46.7% | 10.5 / 10.3 | 15 | N156K (AAC→AAA) | yiiQ | YiiQ family protein |
| Reads supporting (aligned to +/- strand): ref base G (8/0); new base T (0/7); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCC > NZ_CP009273/4101600‑4101762 | gAAGGTCGCCCCTTTTTCGCCTTTGTCCGCGAAAACATACCGCAGTGCACCTTTTGTCTGGGTGTAATAACGTTTTTTTTTCCCCGCCGt < 1:55663/90‑1 (MQ=255)gAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGt > 2:346879/1‑90 (MQ=255) gCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCg > 2:55663/1‑90 (MQ=255) gCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGttt > 1:198271/1‑90 (MQ=255) aTACCGCAGTCCCCCTTCTGTCTGGGTTTAATACCTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGtttt < 1:258596/90‑1 (MQ=255) aaCGCAGTCCCCTTTTTGTCTGGGTTTAATATTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTgg < 2:49392/90‑1 (MQ=255) aaCGCAGTCCCCCTTCTGTCTCGGTGTAATAACTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTgg < 1:175360/90‑1 (MQ=255) gTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTa > 1:127419/1‑90 (MQ=255) gTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTa > 1:571939/1‑90 (MQ=255) cccccTTCTGTCTCGGTGTAATATTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAAt < 2:488285/87‑1 (MQ=255) tctgtctgGGTGTAATATTTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTg < 1:456987/90‑1 (MQ=255) tctgtctgGGTGTAATAACTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTg < 1:181806/90‑1 (MQ=255) tGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAg > 2:521962/1‑90 (MQ=255) cGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGc > 1:56960/1‑90 (MQ=255) gtgtAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCgg > 2:577861/1‑90 (MQ=255) gtttttttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGttt < 2:329659/54‑1 (MQ=255) gtttttttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTc < 1:307578/59‑1 (MQ=255) gtttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGttt > 1:329659/1‑54 (MQ=255) gtttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTc > 2:307578/1‑59 (MQ=255) gtttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg > 2:220515/1‑90 (MQ=255) gtttgtttTTCCCCACCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg > 2:522322/1‑90 (MQ=255) ttttttttTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGc < 1:521962/90‑1 (MQ=255) ttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGcc > 1:567247/1‑90 (MQ=255) | GAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCC > NZ_CP009273/4101600‑4101762 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |