| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,429,984 | 0 | G | A | 56.2% | ‑2.1 / 16.8 | 16 | intergenic (‑108/+33) | uspF/ompN | universal stress protein UspF/porin OmpN |
| Reads supporting (aligned to +/- strand): ref base G (7/0); new base A (0/9); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGAT > NZ_CP009273/1429897‑1430064 | ccGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaa > 1:26392/1‑90 (MQ=255)ccGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGaa > 2:227991/1‑90 (MQ=255) aaGTGAAACACAAAATAACATTTTTTGTATAAGGACGAATTTCACATTTTTAATTAAAAACAAAGGAGAAAAAAAAAGAAAAAAAAGccc < 2:291792/90‑1 (MQ=255) caaGATGACTTTTTTTGTGTAATGACGGATTTCACTTTTTTAATTTAGAAAAAAGGAGAAAAAAAAAGCAAAAAAAGCCCGCCATAACAg < 1:151065/88‑1 (MQ=255) aGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCg > 1:422734/1‑90 (MQ=255) ttttttGTGTAATGACGGAGTTCACTTTTTTAATTTAGAAAAAAGGAGGAAGAATAAGAAAAAAAAGCCCGCCATAACAGCGGGCaggag < 1:187677/89‑1 (MQ=255) ttttttGAGTAAGGACGGACTTCATTTTTTTAATTAAAAAAAAAGGAGAAAAAAAAAGAAAAAAAGGCCCGCCATAACAGCGGGCaggag < 2:375938/89‑1 (MQ=255) cTTTTTGTGTAATGACGGATTTCACATTTTTAATTTAGAACAAAGGAGAAAAAAAAAGAAAAAAAACCCCGCCATAACAGCGGGCaggag < 1:193736/90‑1 (MQ=255) gtAAGGCCGGAGTTCCCTTTTTTAATTAAAAAAAAAGGAGAAAAAAAAAAAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGa < 2:332561/90‑1 (MQ=255) aaaaTGACGGATTTCTTTTTTTTAATTAAAAAAAAAGGAGAAAAAAAAAAAAAAAAACCCCCGCCATAACAGCGGGCAGGAGGATTTAGa < 2:409581/88‑1 (MQ=255) aTGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACt > 2:73292/1‑90 (MQ=255) aCGGATTTCAATTTTTTAATTTAAAAAAAAGGAGAAAAAAAAAGAAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGAt < 1:73292/90‑1 (MQ=255) ttttAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTaa > 1:386272/1‑90 (MQ=255) gaaTAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGAt > 1:311607/1‑90 (MQ=255) tAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAgaga > 2:120028/1‑86 (MQ=255) aaaGCAAAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAgaga < 1:120028/85‑1 (MQ=255) | CCGCATAAAGTGTAACGCCAGATGACACTTTTTGTGTAATGACGGAGTTCACATTTTTAATTTAGATCAAAGGAGGAAGAATAAGCAGAAAAAGCCCGCCATAACAGCGGGCAGGAGGATTTAGAACTGATAAACCAGACCTAAAGCGACAATATCATCGGTAGAGAT > NZ_CP009273/1429897‑1430064 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |