Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,910	T→G	W30G (TGG→GGG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,910	0	T	G	100.0%	96.2 / NA	30	W30G (TGG→GGG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/13);  total (17/13)

CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC  >  NZ_CP009273/49828‑49991                                                                                   |                                                                                  cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTaa                                                                            >  2:461328/1‑90 (MQ=255)       gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                      <  1:598916/90‑1 (MQ=255)        aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAac                                                                     <  1:62/90‑1 (MQ=255)             ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGGTTAAACGCAACACCtt                                                                >  2:111367/1‑90 (MQ=255)                              gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGt                                                    <  1:552842/85‑1 (MQ=255)                              gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGt                                                    >  2:552842/1‑85 (MQ=255)                              gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                               >  2:564080/1‑90 (MQ=255)                              gcgTTATCGGCATGGAAAACCCCATGCCGGGGGACCCGCCTGCCGATTTCGCCGGGGTTTAACGCAACACCTTAAATAAAACCCGGAttt                                               >  2:352963/1‑89 (MQ=255)                                 ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                            <  1:461328/90‑1 (MQ=255)                                  tATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                           <  1:564080/90‑1 (MQ=255)                                  tATCGGCAGGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                           <  1:610016/90‑1 (MQ=255)                                    tCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgcc                                         >  2:572277/1‑90 (MQ=255)                                        cATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                            <  1:23607/83‑1 (MQ=255)                                        cATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                            >  2:23607/1‑83 (MQ=255)                                         aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                    >  2:50633/1‑90 (MQ=255)                                             aaaaCGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                                <  1:100282/90‑1 (MQ=255)                                                aCGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                      >  1:620370/1‑49 (MQ=255)                                                aCGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                      <  2:620370/49‑1 (MQ=255)                                                 cGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                            >  2:145759/1‑90 (MQ=255)                                                     aTGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca                        >  1:297625/1‑90 (MQ=255)                                                      tGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa                       >  1:146191/1‑90 (MQ=255)                                                                ccgccctgccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTcc             <  2:520120/86‑1 (MQ=255)                                                                 ctgcctgcCGATCTCGCCGGGTTTAAACGCAACACCt                                                                 >  2:386788/1‑37 (MQ=38)                                                                 ctgcctgcCGATCTCGCCGGGTTTAAACGCAACACCt                                                                 <  1:386788/37‑1 (MQ=38)                                                                 ctgcctgcCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg            >  2:84867/1‑90 (MQ=255)                                                                  tgcctgccGATCTCGCCGGGTTTAAACGCAACACCt                                                                 <  2:414005/36‑1 (MQ=38)                                                                  tgcctgccGATCTCGCCGGGTTTAAACGCAACACCt                                                                 >  1:414005/1‑36 (MQ=38)                                                                    cctgccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTg         >  2:250978/1‑90 (MQ=255)                                                                       gccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCa      >  2:558005/1‑90 (MQ=255)                                                                           aTCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc  <  1:558005/90‑1 (MQ=255)                                                                                   |                                                                                  CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC  >  NZ_CP009273/49828‑49991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑