| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,012,922 | 0 | T | G | 53.8% | 11.1 / 14.8 | 13 | S135A (TCG→GCG) | ssnA | putative aminohydrolase SsnA |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base G (7/0); total (9/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCG‑CTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTG > NZ_CP009273/3012835‑3012991 | aGCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCg < 2:245076/90‑1 (MQ=255) gCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGgcg‑c > 2:8960/1‑90 (MQ=255) gCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTc > 1:263063/1‑88 (MQ=255) gCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTc < 2:263063/88‑1 (MQ=255) gCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCgg > 2:59054/1‑89 (MQ=255) gATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGGG‑CGCTCcac > 1:218601/1‑90 (MQ=255) gATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGGG‑CTCTCCCCATTTCGCGGCG‑CAttttt > 1:34442/1‑90 (MQ=255) ggATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCG‑CTCCCCCCCTTTCGCGACG‑CATTTTTTAAAATTGGc > 2:122737/1‑90 (MQ=255) ggTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCg < 1:227187/43‑1 (MQ=255) ggTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCg > 2:227187/1‑43 (MQ=255) ggTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGGG‑CTCTCCCCACTTCGCGACGCCTTTTTTTAAAATTGG‑CTGcgcgcggg > 2:11265/1‑88 (MQ=255) ggTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGCCCCCCCCCATTCCCCGCGC‑CCTTTTTTAAAATTGG‑CTGcgcgcggg > 2:128002/1‑88 (MQ=255) ggTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCG‑CTCTCCCCACTTCGCGCCC‑CCCTTTTTAAAAATGGGCTGcccgcggg > 1:272756/1‑88 (MQ=255) ctcCGGCGTATATCGGCGGGTCG‑CTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTg < 1:94564/90‑1 (MQ=255) | AGCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCG‑CTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTG > NZ_CP009273/3012835‑3012991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |