| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,514,110 | 0 | G | T | 53.8% | 7.5 / 15.9 | 13 | T90K (ACA→AAA) | hofP | DNA utilization protein HofP |
| Reads supporting (aligned to +/- strand): ref base G (1/5); new base T (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGCGGTTCGCA‑GTTTGTCCCGGTACCCAGCGTTAGTACGTCTGGCGTCAGCTGTAAAATTGTCCAGCCGTTTTCCAGCACATCGTTTTGCTGC > NZ_CP009273/3514023‑3514187 | gTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGCGGTTCGCC‑GTTTTTc > 1:165558/1‑90 (MQ=255) cAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGCGGTTCGCA‑GTTTGTCCCgg < 1:317009/90‑1 (MQ=255) aTCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGCGGTTCGCAGGTTTTTCCCggacc > 2:140740/1‑87 (MQ=255) tCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGCGGTTCGCA‑GTTTGTCCCGGTAccc < 1:140740/90‑1 (MQ=255) tCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGCGGTTCGCA‑GTTTGTCCCGGTAccc < 2:59537/90‑1 (MQ=255) gtGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGCGGTTTTCA‑GTTTGTGCCTGTAACCCGCGATact > 2:7637/1‑88 (MQ=255) gCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGCGGTTCGCC‑GTTTTTCCCCGTACCCCGCGTTAGTACGTc > 2:274798/1‑90 (MQ=255) ttGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGCGGTTCGCA‑GTTTTTCCCGGTACCCCGCGTTAGTACGTc > 1:278043/1‑82 (MQ=255) ttGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGCGGTTCGCA‑GTTTGTCCCGGTACCCAGCGTTAGTACGTc < 2:278043/82‑1 (MQ=255) tCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGCGGTTCGCA‑GTTTTTCCCCGTACCCCACGGTGGTACGCCTGGCGTCCGCTGt > 2:210219/1‑90 (MQ=255) ttGCCGTTGCCACAACCATTGTGGCGGTTCGCA‑GTTTTTCCCCGTACCCCGCGTTTGTACGTCTGGGGGCCGCTGTAAAAATTTCCCGcc > 2:308424/1‑90 (MQ=255) aCCATTGTGGCGGTTCGCA‑GTTTGTCCCGGTACCCAGCGTTAGTACGTCTGGCGTCAGCTGTAAAATTGTCCAGCCGTTTTCCAGCACAt < 2:182100/90‑1 (MQ=255) ggTTCGCA‑GTTTTTCCCGGTACCCAGCGTTAGTACGTCTGGCGTCAGCTGTAAAATTGTCCAGCCGTTTTCCAGCACATCGTTTtgctgc > 1:259177/1‑90 (MQ=255) | GTATCAGCATCAACAGTAGTGCGGCTATCCATTGCTTCATTTGTATCTCCTTGCCGTTGCCACAACCATTGTGGCGGTTCGCA‑GTTTGTCCCGGTACCCAGCGTTAGTACGTCTGGCGTCAGCTGTAAAATTGTCCAGCCGTTTTCCAGCACATCGTTTTGCTGC > NZ_CP009273/3514023‑3514187 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |