| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 115,871 | 0 | T | G | 52.9% | 11.5 / 19.7 | 17 | V35G (GTG→GGG) | ampE | beta‑lactamase regulator AmpE |
| Reads supporting (aligned to +/- strand): ref base T (0/8); new base G (9/0); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTA > NZ_CP009273/115786‑115959 | ttACTGGTGTTAATTTGCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGGGaaa > 2:206744/1‑90 (MQ=255) ggTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGGGAAAAAttt > 2:216890/1‑90 (MQ=255) tgtTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGGGAAAAAttttt > 1:215711/1‑90 (MQ=255) tAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAAAAAttttttt > 2:201337/1‑88 (MQ=255) tCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGTGAAACATTTTTCTCTCGGgc < 1:194932/90‑1 (MQ=255) gAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGTGAAACATTTTTCTCTCGGgcgc < 1:201337/90‑1 (MQ=255) gggCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGGGGAAAATTTTTTTCTCGGGCGCACGTTAGGGATGACCat > 2:17670/1‑90 (MQ=255) ggCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGGGGAACATTTTTTTCTTGGGGGCACGCTAGGGCTGAACatt > 2:49453/1‑90 (MQ=255) ggCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGGGGAAAATTTTTTTTTCGGGCGCCCGGTTGGCATGACCatt > 2:27615/1‑90 (MQ=255) cTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTg < 1:17670/90‑1 (MQ=255) gTCTTGAAGCGTTCTTTCGGC‑GGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTAc < 2:220682/90‑1 (MQ=255) gTTCTTTCGGC‑GGGGGAAACATTTTTTTCTCGGGGGCACGTTAGGCATGACCATTATTGCGGTGGGGGGGACTTTTTTTCTGTTACgcgc > 1:209955/1‑90 (MQ=255) tttCGGC‑GGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTg < 1:19083/90‑1 (MQ=255) cggc‑ggGGGAAAAATTTTTTTCTCGGGGGCACGTTAGGCATGACCATTTTTTCGATGGGGGTGACTTTTTTTCTTTTTCGCGCATTTCAg > 2:19083/1‑90 (MQ=255) gc‑ggGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAggg < 2:52089/90‑1 (MQ=255) ggTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGt < 1:165994/90‑1 (MQ=255) gTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTa < 1:227896/90‑1 (MQ=255) | TTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGC‑GGGTGAAACATTTTTCTCTCGGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTA > NZ_CP009273/115786‑115959 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |