| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,531,777 | 0 | T | G | 53.8% | 9.2 / 11.8 | 13 | V345G (GTC→GGC) | yhgF | Tex family protein |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGC > NZ_CP009273/3531701‑3531863 | aTGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGc < 1:111046/90‑1 (MQ=255) tGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGc < 2:21989/90‑1 (MQ=255) cTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGcgaccgac < 1:241637/90‑1 (MQ=255) gCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGGGGGCGGTGCCCCTGGCAAACTGGGGGGGACCGacac > 1:196313/1‑90 (MQ=255) gCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGGGGGCGGTGCCACCGGCAAAATGGGGGGGGCCGgcac > 2:54965/1‑90 (MQ=255) tGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCAt < 2:269879/90‑1 (MQ=255) gggCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGGCGATGCCCCTGGCAAACTGGGGGCGACCGACACCATTTACCCCca > 1:23988/1‑90 (MQ=255) gggCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGGCGATGCCACCCGCAAACTGGGGGGGGCCGCCACCCTTTTcccccc > 1:157418/1‑87 (MQ=255) ccTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGGGGGCGATGCCACCGGCAAACTGGGGGGGACCGACACCCTTTTCCCCCacac > 1:123272/1‑90 (MQ=255) gCGTACTGGGGTAAAAGTGGCGGTGGGCGATGCCACTGGCAAACTGGGGGGGGCCGACACCATTTACCCGCACACCCGACAGGCCGCaaa > 2:103863/1‑90 (MQ=255) gCGTACTGGGGTAAAAGTGGCGGGGGGGGATGCCCCCGGCAAACTGGGGGGGGCCGACACCACTTTCCCCCACCCCCGGCAGGCCGCcaa > 2:239019/1‑90 (MQ=255) aaaGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGAcc < 2:196313/90‑1 (MQ=255) ggCGGTGGTCGATGCCACTGGCAAAATGGTGGGGACCGACACCATTTACCCCCACACCCGACAGGCCGCAAAAACAGCGGTGACCGTtgc > 2:65074/1‑90 (MQ=255) tggtCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTgcgc < 2:23988/90‑1 (MQ=255) | ATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGC > NZ_CP009273/3531701‑3531863 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |