| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,058,940 | 0 | T | A | 58.3% | ‑1.5 / 10.8 | 12 | T63S (ACC→TCC) | yihO | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base T (4/1); new base A (0/7); total (4/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAG > NZ_CP009273/4058851‑4059026 | atatAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGt > 1:89569/1‑90 (MQ=255) aaataaaGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaaaaa > 1:342/1‑90 (MQ=255) aaGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTc > 1:183385/1‑90 (MQ=255) aaGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTc > 2:169721/1‑90 (MQ=255) aaTTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGa < 1:789/90‑1 (MQ=255) gggagtagagaAAAAACCCGGTGAGCATATGGGAAAACGCGGAAAAAAACTCCCCCCCCAAAAAAATAATCCCGCCATAGTAAGCAGGCa < 1:135953/88‑1 (MQ=255) gcgAGTCGAGAAAAAACCCGGGGACCATACCGAAAAACGGGAAAAAAACCTCCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCa < 2:146838/90‑1 (MQ=255) aaaTCCGGTGACCATATCGGAAACCGCGGAAAAAACCTCCCCCCCAAAAAAAAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATc < 2:89569/90‑1 (MQ=255) aaTCCGGTGAGCATATCGGAAAACGCGGAAAAAAACTTCGCCACCAAAAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCg < 1:110996/90‑1 (MQ=255) tCGGAAAACGCGGAAAAAAACTTCCCCACAAAAAAAATAATCCCGCCATAGTAAGCAGGCATCACTAATTCATCGGTATAAAATTTgagg < 1:169721/90‑1 (MQ=255) gggaaaaCGCGAAAAAAACCTCCCCCCCAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagga < 2:2208/86‑1 (MQ=255) cGGTAAACGCGGAAAAAACCTTCCCCACCAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagga < 1:82883/90‑1 (MQ=255) cgGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACagag > 2:14962/1‑90 (MQ=255) | ATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAG > NZ_CP009273/4058851‑4059026 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |