| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,292,475 | 0 | T | G | 53.8% | 11.5 / 14.3 | 14 | T141P (ACC→CCC) | mdtO | multidrug efflux transporter permease subunit MdtO |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (7/0); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATC > NZ_CP009273/4292388‑4292563 | tcaGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGttt < 1:140995/90‑1 (MQ=255) tGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAAt < 2:135336/90‑1 (MQ=255) ggCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTTCCCGGAAAAAACGGCaa > 1:147198/1‑90 (MQ=255) acGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGGTTGCCCCGAAAAAACGACAAAGGCGACg > 1:272928/1‑90 (MQ=255) accacAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGaaaa < 2:48331/90‑1 (MQ=255) ccacAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGGTTGCCCCTAAAAAACGACAAAGGGGGCGGGGgaaaa > 2:47427/1‑90 (MQ=255) acaGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTGCCCCCAAAAAACGACAAAAGGGACGGCGgaaaaaa > 2:131070/1‑88 (MQ=255) gCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAAGGTTGCCCCCAAAAAACGCCAAAGGCGGCGGCGGAAAAGAACa > 2:261814/1‑90 (MQ=255) gTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGGTTGGCCCTAAATAGCGACAACGGCGCCGGCGCAAAAAAcccac > 1:220744/1‑87 (MQ=255) ggATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATc < 1:173988/90‑1 (MQ=255) gTCGAGCATGGCGGGGGAGGGTTGGCCGTAAAAAGCGACAAAGGGGGCGGCGGAAAAAAACAGCCCCCAGCGGGGGGGGGGGCTCaaaaa > 2:173988/1‑90 (MQ=255) gTCGAGCATGGCGGGGAAGGATTTTCCCCAAATAGCGACAATAGGGGCGGCGAAAAAAAACGACCCCAACCGAGGGGGGTGCGACaaaaa > 2:166046/1‑90 (MQ=255) tGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGc < 1:189246/90‑1 (MQ=255) tGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGc < 1:234672/90‑1 (MQ=255) gTTTGCCCGTAAATAGCGACAATGGCGACGACGAAAAAGACCACCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATc < 2:114766/90‑1 (MQ=255) | TCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATC > NZ_CP009273/4292388‑4292563 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |