Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	726,489	A→G	E484G (GAA→GGA)	rhsC →	rhs element protein RhsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	726,489	0	A	G	100.0%	32.3 / NA	11	E484G (GAA→GGA)	rhsC	rhs element protein RhsC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/6);  total (5/6)

CATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACC  >  NZ_CP009273/726403‑726556                                                                                       |                                                                    cATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGGAAt                                                                  <  2:241005/90‑1 (MQ=255)       cacGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGGAATACGCCg                                                            <  1:12694/90‑1 (MQ=255)                tGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGGAATACGCCGGGAATatga                                                   <  1:58062/90‑1 (MQ=255)                  gCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGGAATACGCCGGGaa                                                        <  1:223186/83‑1 (MQ=255)                  gCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGGAATACGCCGGGaa                                                        >  2:223186/1‑83 (MQ=255)                       gCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGGAATACGCCGGGAATATGATGAATgg                                            >  1:262165/1‑90 (MQ=255)                       gCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGGAATACGCCGGGAATATGATGAATgg                                            >  2:180079/1‑90 (MQ=255)                          tCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGGAATACGCCGGGAATATGATGAATGGGGc                                         <  1:52230/90‑1 (MQ=255)                                                                 gCCACCGGGCCTGACGGGCTGGGAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCAcc  >  2:129842/1‑90 (MQ=255)                                                                      cGGGCCTGACGGGCTGGGAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAAc                        <  1:228723/63‑1 (MQ=255)                                                                      cGGGCCTGACGGGCTGGGAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAAc                        >  2:228723/1‑63 (MQ=255)                                                                                       |                                                                    CATCACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAGCCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAAATACGCCGGGAATATGATGAATGGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACC  >  NZ_CP009273/726403‑726556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑