Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,598,4940CT54.5% 0.5 / 10.9 11S64L (TCG→TTG) lsrDautoinducer 2 ABC transporter permease LsrD
Reads supporting (aligned to +/- strand):  ref base C (0/5);  new base T (6/0);  total (6/5)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTG  >  NZ_CP009273/1598416‑1598579
                                                                              |                                                                                     
gCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTTGTTTGa                                                                            >  1:11143/1‑90 (MQ=255)
     aGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTGTTTTGTTTGACCATc                                                                       >  2:107102/1‑90 (MQ=255)
         aCTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACTATCGGcc                                                                   <  1:145589/90‑1 (MQ=255)
         aCTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGcc                                                                   <  1:107102/90‑1 (MQ=255)
                    attggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTGGTTCGACCATCGGCCTCTGCGCcatt                                                        >  1:152779/1‑90 (MQ=255)
                        gcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTTGTTCGACCATCGGCCCCTGCGCcattggat                                                    >  1:22079/1‑90 (MQ=255)
                            tgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTGGTTCTGCCATCGGCCTCTTCGGCCTTGCATTtgg                                                >  1:363156/1‑90 (MQ=255)
                                 cccTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTAc                                           <  2:44951/90‑1 (MQ=255)
                                                           aGTGGCGGGGTCGATATTTTGTTTTGTTCGACCATCGGCCTCTGCGCCCTTGCATTTGGGGGACTGTTTTTAAATGGGGTGGCCATGCCg                 >  2:33280/1‑90 (MQ=255)
                                                                  ggATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGa          <  1:316427/90‑1 (MQ=255)
                                                                          atTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTg  <  2:211884/90‑1 (MQ=255)
                                                                              |                                                                                     
GCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTG  >  NZ_CP009273/1598416‑1598579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑