Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,334,2240TA57.1% 1.2 / 14.9 14D1142V (GAT→GTT) yfaLAIDA‑I family autotransporter adhesin YfaL/EhaC
Reads supporting (aligned to +/- strand):  ref base T (1/5);  new base A (8/0);  total (9/5)
Fisher's exact test for biased strand distribution p-value = 3.00e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCAC  >  NZ_CP009273/2334144‑2334281
                                                                                |                                                         
aTTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGGAAAAACATcctcct                                                  >  2:366024/1‑90 (MQ=255)
aTTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGGAAAAAAATCctgct                                                  >  2:67168/1‑90 (MQ=255)
    gCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCAc                                              <  1:79465/90‑1 (MQ=255)
                 aGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAAACATCCTGCTGCACGCCCTGATAAATc                                 >  1:163458/1‑90 (MQ=255)
                 aGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGGAAAAACATCCTGCTGCCCCCCCCGATAAATc                                 >  2:323415/1‑90 (MQ=255)
                               atcatcACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCgg                   <  2:220995/90‑1 (MQ=255)
                                   tcaCCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCa               <  2:248526/90‑1 (MQ=255)
                                     aCCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAAACATCCTGCTGCACGCCCTGATAAATCACCCCCGCCCGCGGTTCAAt             >  2:177/1‑90 (MQ=255)
                                     aCCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAAAAATCCTTCTTCCCGCCCCTATAAAAAACCTTCGCCCGCGGGTCAAt             >  2:333460/1‑90 (MQ=255)
                                          gCGATTGTGACACGCGCGCACGGTTAGCGGCGGGAAAAAAATCCTTCTTCACGCCCCGGTAAAACACAGGCGCCCCCGGGTTAATcccca        >  2:32671/1‑90 (MQ=255)
                                           cGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAAACATCCTGCTGCCCGCCCCGATAAAACACCCGCGCCTGCGGGTCAATcaccac       >  2:334817/1‑90 (MQ=255)
                                                gtgACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCaccacaccac  <  2:148102/90‑1 (MQ=255)
                                                       gcgcgcACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTg                                        >  1:365090/1‑45 (MQ=255)
                                                       gcgcgcACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTg                                        <  2:365090/45‑1 (MQ=255)
                                                                                |                                                         
ATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCAC  >  NZ_CP009273/2334144‑2334281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑