| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,955,854 | 0 | T | G | 54.5% | 6.6 / 10.6 | 11 | S90S (TCA→TCC) | ppdC | prepilin‑type N‑terminal cleavage/methylation domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (3/2); new base G (6/0); total (9/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGC > NZ_CP009273/2955774‑2955940 | tAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGGCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAGc > 1:338177/1‑90 (MQ=255) gcccTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTa < 1:46334/89‑1 (MQ=255) cccTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCCTCCCTGCCCCCCGGGGGAACTAACGGaa > 2:210440/1‑90 (MQ=255) ttAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCCGGGGAAACTAGCGTAACGc > 2:8323/1‑90 (MQ=255) aGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCCGGGGAAACTAGCGTAACGCTGATGCt > 1:363382/1‑90 (MQ=255) aGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGGCATCTCCCCTTCTCTTCCCCCCGGGGAAAATAGCGGAACGCTGATGcc > 2:46334/1‑89 (MQ=255) tCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAGCGTAACGCTGATGCTGAcaca > 1:300205/1‑90 (MQ=255) ttCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTg < 1:20621/46‑1 (MQ=255) ttCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTg > 2:20621/1‑46 (MQ=255) tctcTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAggg > 2:290042/1‑89 (MQ=255) gggTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATcgc > 2:288145/1‑90 (MQ=255) | TAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGC > NZ_CP009273/2955774‑2955940 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |