Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,341,2370TG54.5% 7.4 / 11.5 11V255G (GTG→GGG) rapZRNase adapter RapZ
Reads supporting (aligned to +/- strand):  ref base T (0/5);  new base G (6/0);  total (6/5)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGT  >  NZ_CP009273/3341157‑3341319
                                                                                |                                                                                  
aTGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGGGTATAtttc                                                                           >  1:7573/1‑88 (MQ=255)
aTGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGGTCGGGGGATATTGc                                                                           >  1:10674/1‑90 (MQ=255)
  ggTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCag                                                                         <  1:266135/90‑1 (MQ=255)
         tATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACt                                                                  <  2:53564/90‑1 (MQ=255)
          aTGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGAACCCGTCGGGGGTTATTGCAGAGCAAccg                                                                 >  2:247003/1‑88 (MQ=255)
            gCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATCGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGc                                                               <  1:75193/90‑1 (MQ=255)
                 aaaCCAACAACCGTAGCTACTTGACGGTCGCCATCGGTTGTACCGGCGGGAAGCACCCTTCGGGGGATATTTCAGAGCAACTGGCAGacc                                                          >  2:75193/1‑89 (MQ=255)
                      aacaacCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGGGTATATTTCAGAGCAAATGGCAGACTACTTc                                                     >  2:138246/1‑90 (MQ=255)
                              tAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGGGTATATTTCAGGGGAACTGGCAGACTACTTCCGCTcgcg                                             >  1:103816/1‑90 (MQ=255)
                                                                    aGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGaaa       <  1:247003/90‑1 (MQ=255)
                                                                         cGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGt  <  2:7573/90‑1 (MQ=255)
                                                                                |                                                                                  
ATGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGT  >  NZ_CP009273/3341157‑3341319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑