| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,538,366 | 0 | A | C | 53.8% | 10.3 / 13.3 | 13 | L42F (TTA→TTC) | gntX | DNA utilization protein GntX |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTAGGTCATTGGGGGATTTGTTCGGTCTGTTCACGCGCCACCCGCACAGATAAAACGTTATGCCCACAATGTGGATTACCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCCCTGGCAAAGACTGGTCACGGTTGCC > NZ_CP009273/3538289‑3538444 | ttAGGTCATTGGGGGTTTTGTTCGGTCTGTTCCCGCGCCCCCCGCACAAAAAAAACGTTATGCCCAAAATGTGGATCACCCGCCacacac < 2:245881/90‑1 (MQ=255) tAGGTCATTGGGGGATTTGTTCGGTCTGTTCACGCGCCACCCGCACAGATAAAACGTTATGCCCACAATGTGGATTACCCGCCACACACt > 2:238076/1‑90 (MQ=255) aTTTGTTCGGTCTGTTCCCGCGCCCCCCGCACAGATAAACCGTTATCCCCACAATGTGGATTCCCCGCCACACACTCCCATCTTCCCTGc < 1:238076/90‑1 (MQ=255) cACCCGCACAGATAAAACGTTATGCCCACAATGTGGATTACCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAAccgcc > 1:340000/1‑90 (MQ=255) cccGCACAGATAAACCGTTATGCCCACAATGTGGATTCCCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACcgccgc < 1:142136/90‑1 (MQ=255) cGCACAGATAAAACGTTATGCCCACAATGTGGATTCCCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGccc < 1:88990/90‑1 (MQ=255) gATAAACCGTTATGCCCACAATGGGGATTCCCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCCCTGGCaa < 2:138048/90‑1 (MQ=255) accGTTATCCCCAAAATGTGGATTCCCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCCCTGGCAAAGACt < 2:100553/88‑1 (MQ=255) tATGCCCACAATGTGGATTACCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCCCTGGCAAAGACTGGTCa > 1:244167/1‑90 (MQ=255) atccccAAAATGTGGATTCCCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCCCTGGCAAAGACTGGTCAc < 1:215577/87‑1 (MQ=255) caATGTGGATTACCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCCCTGGCAAAGACTGGTCACGGTTGcc > 1:99792/1‑90 (MQ=255) tgGATTCCCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCCCTGGCAAAGACTGGTCAc < 1:349022/78‑1 (MQ=255) tgGATTACCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCCCTGGCAAAGACTGGTCAc > 2:349022/1‑78 (MQ=255) | TTAGGTCATTGGGGGATTTGTTCGGTCTGTTCACGCGCCACCCGCACAGATAAAACGTTATGCCCACAATGTGGATTACCCGCCACACACTCCCATCTTCCCTGCGGTCGCTGCCTGCAAAAACCGCCGCCCTGGCAAAGACTGGTCACGGTTGCC > NZ_CP009273/3538289‑3538444 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |