| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,571,647 | 0 | T | C | 54.5% | 1.4 / 11.0 | 11 | E147G (GAA→GGA) | gntR | gluconate operon transcriptional repressor GntR |
| Reads supporting (aligned to +/- strand): ref base T (3/2); new base C (0/6); total (3/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.06e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAG > NZ_CP009273/3571565‑3571736 | ggCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCcaccac < 1:19734/90‑1 (MQ=255) cATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATAcc > 2:333211/1‑90 (MQ=255) gctgctTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCcaccac < 1:261066/71‑1 (MQ=255) gctgctTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCcaccac > 2:261066/1‑71 (MQ=255) gctTAAAATTTACAAAACCCGACGGCAAAATAAAGGCAGGGCCATTTGCTGCCCATCATTCCCCCCACGGAAATCCCCGCCACTTCAatc < 2:239727/90‑1 (MQ=255) gACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCgt > 1:120922/1‑90 (MQ=255) acaaaGGCAGGGCGTTTTCCTGCCCATCAGTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACg < 2:31198/87‑1 (MQ=255) ggCAGGGCGTCTTGCTCCCCACCAGTCCCCCCAGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCgg < 2:303249/90‑1 (MQ=255) atttGCTGCCCATCAGTCCCCCCACGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCa < 1:167498/88‑1 (MQ=255) catcatTCCCACCAGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGat < 2:178046/90‑1 (MQ=255) cATCAGTCCCCCCAGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGat < 1:35691/90‑1 (MQ=255) tCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAg > 1:164961/1‑90 (MQ=255) | GGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAG > NZ_CP009273/3571565‑3571736 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |