Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,195,5420CA55.6% 2.4 / 10.6 9A106A (GCG→GCT) purDphosphoribosylamine‑‑glycine ligase
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (5/0);  total (7/2)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GATACGCCAGCGCAGGTTCTACCTCGGTGAAGTTCTGGTATTCCGCCGTAGGGATCTTATGGCGGGCCAGGAAATCTTTGGTAAACGCTTTTGAGCCTTCCAGTTGGGCCGCACCTGCGGTTGGGCCGAAGATTTTCAGCCCGGCGGCGCGGAAGGTATCG  >  NZ_CP009273/4195457‑4195617
                                                                                     |                                                                           
gATACGCCAGCGCAGGTTCTACCTCGGTGAAGTTCTGGTATTCCGCCGTAGGGATCTTATGGCGGGCCCGGAAAACTTTTGGAAAAGCtt                                                                         >  1:321824/1‑90 (MQ=255)
                      cTCGGTGAAGTTCTGGTATTCCGCCGTAGGGATCTTATGGCGGGCCAGGAAATCTTTGGTAAACGCTTTTGAGCCTTCCAGTTGGGCCGc                                                   <  1:356200/90‑1 (MQ=255)
                        cGGTGAAGTTCTGGTATTCCGCCGTAGGGATCTTATGGCGGGCCCGGAAAACTTTTTTAAAAGCCTTTTTGCCTTCCCGTTGGGgcgccc                                                 >  2:318631/1‑88 (MQ=255)
                                  cTGGTATTCCGCCGTAGGGATCTTATGGCGGGCCAGGAAAACTTTTGTAAAAACTTTTTTGCCTTCCCGTTTGGGCGCCCCCGCGGGTgg                                       >  2:116148/1‑90 (MQ=255)
                                         tCCGCCGTAGGGATCTTATGGCGGGCCCGGAAATCTTTTGGAAAAGCTTTTTAGCCTTCCAGTTGGGCCGCCCCCGCGGGTGGGGCGGAg                                >  1:198180/1‑90 (MQ=255)
                                               gTAGGGATCTTATGGCGGGCCCGGGAAACTTTTGTAAAAGCTTTTTAGCCTTCCCGTTGGGGCGCCCCCGCGGGTGGGGCGGAGAatttt                          >  2:120328/1‑89 (MQ=255)
                                                               gggCCAGGAAATCTTTGGTAAACGCTTTTGAGCCTTCCAGTTGGGCCGCACCTGCGGTTGGGCCGAAGATTTTCAGCCCGGCGGCGCGGa          >  1:10617/1‑90 (MQ=255)
                                                                ggCCAGGAAATCTTTGGTAAACGCTTTTGAGCCTTCCAGTTGGGCCGCACCTGCGGTTGGGCCGAAGATTTTCAGCCCGGCGGCGCGGaa         <  2:10617/90‑1 (MQ=255)
                                                                       aaaTCTTTGGTAAACGCTTTTGAGCCTTCCAGTTGGGCCGCACCTGCGGTTGGGCCGAAGATTTTCAGCCCGGCGGCGCGGAAGGTATCg  >  2:165948/1‑90 (MQ=255)
                                                                                     |                                                                           
GATACGCCAGCGCAGGTTCTACCTCGGTGAAGTTCTGGTATTCCGCCGTAGGGATCTTATGGCGGGCCAGGAAATCTTTGGTAAACGCTTTTGAGCCTTCCAGTTGGGCCGCACCTGCGGTTGGGCCGAAGATTTTCAGCCCGGCGGCGCGGAAGGTATCG  >  NZ_CP009273/4195457‑4195617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑