| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,292,471 | 0 | A | G | 53.3% | 8.4 / 16.7 | 15 | F142S (TTC→TCC) | mdtO | multidrug efflux transporter permease subunit MdtO |
| Reads supporting (aligned to +/- strand): ref base A (2/5); new base G (8/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGC > NZ_CP009273/4292385‑4292552 | tcatcaGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAgg > 2:69869/1‑90 (MQ=255) tGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAAt > 1:102998/1‑90 (MQ=255) gagGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTTCCCGGAAAAAACGAc > 2:63072/1‑90 (MQ=255) aacGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGGGGGTTGCCCCCAAAAAACGACAAAGGCGAc > 2:222139/1‑90 (MQ=255) gATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGc < 2:102998/90‑1 (MQ=255) caccacAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTGGCCGTAAAAAACGACAAAGGGGGCGGGGaaa > 2:5541/1‑90 (MQ=255) ccacAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTGCCCGTAAAAAACGACAAAGGGGGCGGCGaaaaa > 1:47576/1‑90 (MQ=255) aaGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTTCCCGTAAATAGCGACAATGGCGACGGGGAAAAAAAACAAcccc > 1:250732/1‑90 (MQ=255) cACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTGGCCGTAAAAAACGACAAAGGGGACGGGGGAAAAAAACAGCCCCCAGCg > 2:50675/1‑90 (MQ=255) cACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGGCGGCGAAAAAAACCAGCCCCCAGCg > 1:326736/1‑90 (MQ=255) cTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTgc < 1:153631/90‑1 (MQ=255) cTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTgc < 1:69869/90‑1 (MQ=255) gATAGTCGAGCATGGCGGGGGAGGGTTTCCCGTAAATAACGACAAAGGCGACGGCGAAAAAAAACAGCCCCCAACGGTGGGGGGGCATCa > 2:4873/1‑90 (MQ=255) tGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGc < 2:324553/90‑1 (MQ=255) tGGCGGGGAAGGTTTGCCCGAAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGc < 2:219495/90‑1 (MQ=255) | TCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGC > NZ_CP009273/4292385‑4292552 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |