| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 240,196 | 0 | A | C | 52.9% | 6.4 / 16.7 | 17 | H56P (CAT→CCT) | lpcA | D‑sedoheptulose 7‑phosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base A (2/6); new base C (9/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCCATTCAGCGCGCGGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGAGTTGACCGGTCGCTACCGTGAAAACCGTCCGGGCTACCCGGCGA > NZ_CP009273/240113‑240267 | cGCCATTCAGCGCGCGGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGa > 1:346949/1‑90 (MQ=255)cGCCATTCAGCGCGCGGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGa < 2:296970/90‑1 (MQ=255) ttCAGCGCGCGGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGGTTCCCTTGCGACGcca > 1:352366/1‑88 (MQ=255) cAGCGCGCGGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGGTCCCCTTTCGACGCTCTg > 1:38227/1‑90 (MQ=255) cgcgGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACtt < 2:92683/90‑1 (MQ=255) cgcgGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACtt < 2:325670/90‑1 (MQ=255) ggTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGa < 2:301354/90‑1 (MQ=255) tCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCCACGCTATGCACTTTGCCGAag < 2:172867/90‑1 (MQ=255) aGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTTCCCTTTCGGCGCTATGCACTTTTTCGAAGAGGTGACCGGgcgc > 2:302396/1‑90 (MQ=255) aGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCCTTTCGACGCCATGCACTTTTCCGCAGAGGTGACCGGTCGc > 2:116949/1‑90 (MQ=255) aGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCCTTTCCACGCGATGGACTTTTCCCCAGAGTTGAACGGgcgc > 2:284562/1‑90 (MQ=255) cTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGAGTTGACCGGTCGCTa < 1:350097/90‑1 (MQ=255) cGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTTCCCTTGCGGCGCCCTTGACTTTTCCGCAAAGGTGGCCCGGCGCTGCCGCGaaaa > 2:194961/1‑90 (MQ=255) ttCCTGCGGCAACGGCGGTTCCCCTTGCGACGCTATGCACTTTGCCGAAGAGGTGACCGGGCGCTACCCTGGAAACCCCCCGGGCTAccc > 2:125214/1‑90 (MQ=255) gCGGCAACGGCGGTTCCCCTTTCGACGCTATGCACTTTTCCGAAGAGGTGACCGGGCGCTCCCCTGAAAAACCTCCCGGCCACCCcgcgg > 2:96975/1‑89 (MQ=255) gCGGCAACGGCGGTTCCCCTTGCGACGCTATGCACTTTGCCGAAGAGTTGACCGGGCGCTACCGTGAAAACCGTCCGGGCTACCCcgcgg > 1:107876/1‑89 (MQ=255) gCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTTCCGAAGAGGTGACCGGGCGCTACCGCGAAAACCCTCCGGGCTACCCcgcgg > 2:110542/1‑89 (MQ=255) | CGCCATTCAGCGCGCGGCGGTCCTGTTAGCAGACAGCTTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATGCACTTTGCCGAAGAGTTGACCGGTCGCTACCGTGAAAACCGTCCGGGCTACCCGGCGA > NZ_CP009273/240113‑240267 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |