Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,613,687	G→T	G104V (GGC→GTC)	marR →	multiple antibiotic resistance transcriptional regulator MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,613,687	0	G	T	100.0%	35.5 / NA	11	G104V (GGC→GTC)	marR	multiple antibiotic resistance transcriptional regulator MarR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (5/6);  total (5/6)

TCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAG  >  NZ_CP009273/1613604‑1613770                                                                                    |                                                                                    tCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcgtcgcggc                                                                               >  2:112158/1‑90 (MQ=255)  cGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCa                                                                              <  1:112158/90‑1 (MQ=255)  cGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCa                                                                              <  1:112627/90‑1 (MQ=255)  cGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCa                                                                              <  2:331900/90‑1 (MQ=255)        gTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATAtgt                                                                        >  2:23673/1‑90 (MQ=255)                 ggCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGc                                                               <  2:260404/90‑1 (MQ=255)                      ggTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCa                                                          >  1:259083/1‑90 (MQ=255)                            aaGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGt                                                    <  1:336518/90‑1 (MQ=255)                                 tGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGcc                                               >  1:207764/1‑90 (MQ=255)                                            aaTGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCAc                                    >  2:199781/1‑90 (MQ=255)                                                              cTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAc                  >  2:145416/1‑90 (MQ=255)                                                                              ccGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAg  <  1:366406/90‑1 (MQ=255)                                                                                    |                                                                                    TCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAG  >  NZ_CP009273/1613604‑1613770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑