| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 642,666 | 0 | A | C | 46.3% | 68.9 / 20.1 | 67 | G100G (GGT→GGG) | citG | triphosphoribosyl‑dephospho‑CoA synthase CitG |
| Reads supporting (aligned to +/- strand): ref base A (29/7); new base C (0/31); total (29/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAAT > NZ_CP009273/642577‑642749 | aGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCa > 2:1050414/1‑90 (MQ=255)aGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCa > 2:457130/1‑90 (MQ=255)aGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCa > 2:1100158/1‑90 (MQ=255) aaaCGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCt > 2:637647/1‑90 (MQ=255) aaaCGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCt > 2:2265337/1‑90 (MQ=255) aaaCGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCt > 2:2358120/1‑90 (MQ=255) ggCCAATTGCCGCACATAGCACCCCAAAAAAAAAAATGCTCCCTTTATGCGTTTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGc < 2:2547566/90‑1 (MQ=255) cAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAg < 1:1050331/90‑1 (MQ=255) cAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAg < 1:1307209/90‑1 (MQ=255) aTTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGcc < 1:403005/90‑1 (MQ=255) aTTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGcc < 1:3219110/90‑1 (MQ=255) aTTGCCGCACATAGCAGCCCAAAAAAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGcc < 2:1645378/90‑1 (MQ=255) aTTGCCGCACATAGCAGCCAAAAAAAAAAAATGCTCCCTTTATGCGTGTTTCCGCCCGCAGTGGCGCGGAACACATCCCCTTCGCAAGcc < 2:1932209/90‑1 (MQ=255) aTTGCCGCACATAGCACCCCAAAAAAAAAAATGCTGCCTTTATGCGTGTTTCCGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGcc < 2:2486729/90‑1 (MQ=255) aTTCCCGCACATAGCAGCCCTAAAAAAAAAATGCTGCCTTTATGCGTTTTTACGCCCGCAGTGGCGCGAAACATATCCCCTTCGCAAGcc < 2:2489970/90‑1 (MQ=255) ttGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCa > 2:2553943/1‑90 (MQ=255) cacagcagcCTTAAAGAAAAAACCCTCCCTTTATGCTTGTTTCCGCCGGCGGTGGCGGGAAAAATATCCCCTTCGCAAGCCATACCAAtt < 2:1059498/87‑1 (MQ=255) caTAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTCCGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAAtt < 1:1282789/90‑1 (MQ=255) caTAGCACCCCTAAAAAAAAAATGCTCCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGAAACATATCCCCTTCGCAAGCCATACCAAtt < 2:1830635/90‑1 (MQ=255) tAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTgg < 1:1113519/90‑1 (MQ=255) tAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTgg > 1:2692406/1‑90 (MQ=255) tAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTgg > 1:1297191/1‑90 (MQ=255) tAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTgg > 2:1526478/1‑90 (MQ=255) cagcCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCg > 1:60293/1‑90 (MQ=255) cagcCCTAAAGAAAAAAGGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCg < 1:2672898/90‑1 (MQ=255) cagcCCAAAAAAAAAAATGCTCCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCg < 2:167116/90‑1 (MQ=255) caccccAAAAAAAAAAATGCTGCTTTTATGCGTTTTTCCGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCg < 2:2042756/87‑1 (MQ=255) gcCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTa > 1:2643645/1‑90 (MQ=255) gcCCTAAACAAAAAATGCTCCCTTTATGCGCGTTTCCGCCCGCAGTGGGGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTa < 1:940356/90‑1 (MQ=255) gcCCTAAAAAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGGGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTa < 2:978134/90‑1 (MQ=255) cccTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTaa < 1:2358120/90‑1 (MQ=255) cccTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTaa < 1:1100158/90‑1 (MQ=255) caaaaGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATc < 1:1940074/88‑1 (MQ=255) tAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATcc > 1:491042/1‑90 (MQ=255) aaaGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCg > 1:1437346/1‑90 (MQ=255) aaaGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCg > 2:2598950/1‑90 (MQ=255) aaaGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCg > 1:1674485/1‑90 (MQ=255) aaaGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCg > 2:160372/1‑90 (MQ=255) aaaGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCg > 2:3104364/1‑90 (MQ=255) aaaGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCg > 2:772182/1‑90 (MQ=255) aaaGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCg > 1:1875653/1‑90 (MQ=255) aaaGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCg > 1:78771/1‑90 (MQ=255) aaaaaaaGGCTTCCTTTATACGTTTTTCCGCCGGGAGTGGCGCGAAACATACCCCCCTCGCAAGCCATACCAATTGGGCGTAATCCGTgg < 2:1437346/89‑1 (MQ=255) aaaaaaTGCTGCCTTTAGGCGTTTTTCCCCCCGGGGGGGGGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGa < 2:1914078/90‑1 (MQ=255) aaaaTGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGt > 1:1288884/1‑90 (MQ=255) tgctgcCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCg > 2:1147837/1‑90 (MQ=255) tgctgcCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCg > 2:3079272/1‑90 (MQ=255) cTTTATGCGTTTTTCCGCCCGGGGGGGCGGGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTg < 2:2814727/90‑1 (MQ=255) cTTTATGCGTGTTTCCCCCCGCAGGGGCGCGAAACATCCCCCCTCCCCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTg < 2:1288884/90‑1 (MQ=255) tGCGTTTTTACGCCCGCAGTGGCGCGAAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGcc < 1:2287387/90‑1 (MQ=255) tGCGTGTTTACGCCCGCAGGGGTGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGcc < 1:1786306/90‑1 (MQ=255) gtgtTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCAtt > 1:1196757/1‑90 (MQ=255) tgtTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCAttt > 2:2401232/1‑90 (MQ=255) ttACGCCCGCAGGGGGGCGGAACATATCACCTTCGCAAGCCATAACAATTGGGCGTAATCCGTGGAGCGCCGCTTCTGGTGCCATTTCCg > 2:3296631/1‑90 (MQ=255) ccGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCa < 1:110792/89‑1 (MQ=255) cGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACa > 2:2634539/1‑90 (MQ=255) gCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGATTCTGGTGCCATTTCCGCACTACAg > 1:1033813/1‑90 (MQ=255) gggTGGCGCGGAACATCCCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAgg < 2:686824/88‑1 (MQ=255) cAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAgg < 1:400876/90‑1 (MQ=255) cAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAgg < 1:3073352/90‑1 (MQ=255) cAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAgg < 2:2509125/90‑1 (MQ=255) gTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCa < 1:2428335/90‑1 (MQ=255) gcgcGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCa < 1:2234960/90‑1 (MQ=255) gcgGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCaaa > 2:2904437/1‑90 (MQ=255) ggaaaCATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAAt < 2:1674485/87‑1 (MQ=255) cgGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGAACCAAAt < 1:1533304/90‑1 (MQ=255) gAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTc > 1:344832/1‑90 (MQ=255) cATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAAt < 1:2894273/90‑1 (MQ=255) atatCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGc < 2:2771305/68‑1 (MQ=255) atatCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGc > 1:2771305/1‑68 (MQ=255) | AGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAAT > NZ_CP009273/642577‑642749 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |