| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 822,955 | 0 | T | G | 46.9% | 75.7 / 14.2 | 65 | T495P (ACC→CCC) | ybhF | ATP‑binding cassette domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (3/31); new base G (30/0); total (34/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.39e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAA > NZ_CP009273/822871‑823044 | cTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAggg > 1:2370953/1‑90 (MQ=255) tCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑Ggggggggggg > 1:3181916/1‑90 (MQ=255) tCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAggggg > 2:685784/1‑90 (MQ=255) aTCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑Ggggggggggggg > 2:2518789/1‑88 (MQ=255) tAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGGAACGc > 1:1720436/1‑90 (MQ=255) tgggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGCAACGCCGGaa > 2:767216/1‑90 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGGAACGCCGGGAg > 1:2454490/1‑90 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGCAACGCCGGGAg > 2:2517594/1‑90 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGCAACGCCGGAAg > 2:3344535/1‑90 (MQ=255) tggtgACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑Cg < 1:537973/90‑1 (MQ=255) gACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGGAACGCCGGGAGG‑CGGGtc > 2:626200/1‑90 (MQ=255) gACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGGAAAGCCGGGAGG‑Cgggtt > 1:2518231/1‑87 (MQ=255) aCCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGGGGGGGGCCACGGCCGAAGG‑CGGGtcg > 1:361552/1‑90 (MQ=255) aTCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTtcgtcg < 1:2483848/90‑1 (MQ=255) gTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGGAAAGCCGGAAGG‑CGGTCCGTCGAGAAAc > 2:1922295/1‑90 (MQ=255) gTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGCAAGGCCCGAAGT‑CGGTTCGTCGAGaaaa > 1:1629216/1‑89 (MQ=255) gTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGCAACGCCGGGAGG‑CGGGTCGTTGAGaaaa > 2:736836/1‑89 (MQ=255) tGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACa < 1:1920557/90‑1 (MQ=255) aCGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGa < 2:2370953/90‑1 (MQ=255) cGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCTGGAAGT‑CGGTTCGTCGAGAAACAGaa < 2:2940759/90‑1 (MQ=255) cTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGt < 2:3311080/90‑1 (MQ=255) tttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGGAACGCCGGCAGG‑CGGGTCGTCGAGGAAAAAAAAGTc > 2:452169/1‑90 (MQ=255) ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTcc < 1:632251/90‑1 (MQ=255) ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTcc < 1:93471/90‑1 (MQ=255) ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTcc < 2:1846310/90‑1 (MQ=255) ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTcc < 2:1827063/90‑1 (MQ=255) ctACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGtt < 2:2554457/90‑1 (MQ=255) ccATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCAt < 2:1602050/90‑1 (MQ=255) ccATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGCAACCCCGGAAGG‑CGGGTCGGGGGGAAAAAAAAAGTCCGGGTCAt > 1:3029294/1‑90 (MQ=255) cATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGCGACCCCCGAAGG‑CGGTTCGTCGGGGAAAAAAAAGTCCGGGTTATg > 2:2545143/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGc < 2:2612405/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGc < 1:557979/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGc < 2:3217263/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGc < 2:1629216/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGGGACGCCGGGAGG‑CGGGTCGTCGGGAAAAAAAAAGTCCGGGTCATGc > 2:1627951/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGGAACGCCGGAAGG‑CGGGTCGTCGGGAAAAAGAAAGTGCGGGTCGTGc > 2:3000799/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGCAACGCCGGAAGG‑CGGTTCGTCGAGAACCAGAATGTCCGGGTGATGc > 2:2921415/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGCAAAGCCGGAAGG‑CGGGTCGTCGGGAAAAAAAAAGTCCCGGTCATgg > 1:436955/1‑89 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGAGGGGGGCAACGCCCGGAGT‑CGGTTTGTCGGGAAAAAAAAAGTCCGGGTTATGc > 1:655271/1‑90 (MQ=255) gCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGGAACGCCGGAAGG‑CGGGTCGGCGGGGAAAAAAAAGGCCGGGTCGTGCAt > 2:2480/1‑90 (MQ=255) cTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGGAACGCCGGGAGT‑CGGGGCGGCGAGAAAAAGAAAGTGCGGGTCCTGCATc > 2:2965580/1‑90 (MQ=255) cTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGGAACGCCGGGAGG‑CGGGTCGTCGGGAAAAAAAAAGTCCGGGTCATGCATc > 1:3296725/1‑90 (MQ=255) cTGTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGGAACGCCGGGAAG‑CGGGTCGTCGGGGAAAAAAAAGGCCGGGTCATGCaac > 2:1681616/1‑88 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGGAACCCCCGGAGT‑CGGGTTGTCGAGGAAAAAAAAGTCCGGGTTCTGCATCa > 1:2302954/1‑90 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGC‑GGGGGGGGGGGGGGACGCCGGGAGG‑GGGGGGGTCGAGAAAAAAAAAGGCCGGGTTCTGCATCAg > 2:3193129/1‑90 (MQ=255) tgtgCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc < 1:489251/90‑1 (MQ=255) tgtgCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc < 1:3223458/90‑1 (MQ=255) tgtgCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc < 2:3013868/90‑1 (MQ=255) tgtgCAGCCAAAATTCACGGC‑GGGGGGGGGGGGGAACGCCGGGAGG‑CGGGTCGTCGAGAAAAAGAAAGTCCGGGTCATGCATCAGCGaaa > 2:2288928/1‑89 (MQ=255) gtgCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACa < 2:1832840/90‑1 (MQ=255) gtgCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACa < 2:552428/90‑1 (MQ=255) gtgCAGCCAAAATTCACGGC‑GGGGGAGGGGGTCAACGCCGGGAGC‑CGGTGCGGCGAGAAACAGAACGACCAGGTCATGCCTCAGCGaaaa > 2:3049508/1‑88 (MQ=255) gCAGCCAAAATTCACGGC‑GGGGGGGGGGGGCAACGCCGGAAGT‑CGGGTCGTCGAGAAACAGAAAGTCCGGGTCATGCATCAACGGAAAgg > 1:2583650/1‑90 (MQ=255) gCAGCCAAAATTCACGGC‑GGGGGAGGGGGGCAACGCCGGGAGT‑CGGTTCGTCGGGAAAAAGAAAGTCCGGTTCATGCACCAGCGGACAgg > 1:1489464/1‑90 (MQ=255) aGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGgcc < 1:718039/90‑1 (MQ=255) aGCCAAAATTCACGGC‑GGGGGGGGGGGGGAACGCCGGGAGG‑CGGGTGGTCCAGAAACAGAAAGTCCGGGTCGTGCAGCAGCGAGCAGgcc > 2:3015878/1‑90 (MQ=255) aGCCAAAATTCACGGC‑GGGGGGGGGGGGCAACGCCGGGAGG‑CGGGTCGTCGAGAAAAAGAAAGTCCGGGTTATTCATCAGCGAACAAgcc > 1:2512919/1‑90 (MQ=255) aGCCAAAATTCACGGC‑GGGCGAAGGGGAAAACGCCGCAAGTACGGTCCTTCGAAAATCAAAAACT‑CGGTTCTTGCTTCAGGGAACaaggc > 1:1094308/1‑86 (MQ=255) gCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGgcca < 2:3181916/90‑1 (MQ=255) aTTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGgccagcgcca < 1:626200/90‑1 (MQ=255) cggc‑ggGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAACCAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 1:2921415/90‑1 (MQ=255) cggc‑ggGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 1:1032951/90‑1 (MQ=255) cggc‑ggGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 2:1489464/90‑1 (MQ=255) cggc‑ggGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 1:3061754/90‑1 (MQ=255) cggc‑ggGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 1:1627951/90‑1 (MQ=255) cggc‑ggGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 1:1576031/90‑1 (MQ=255) ggc‑ggGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCt < 2:3265153/90‑1 (MQ=255) tGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTaa < 2:1199960/90‑1 (MQ=255) | CTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGC‑GGGTGAGGGGGTCAACGCCGGAAGT‑CGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAA > NZ_CP009273/822871‑823044 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |