Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,125,8350AC50.7% 75.1 / 86.9 69V162G (GTA→GGA) flgAflagellar basal body P‑ring formation chaperone FlgA
Reads supporting (aligned to +/- strand):  ref base A (25/9);  new base C (0/35);  total (25/44)
Fisher's exact test for biased strand distribution p-value = 1.26e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.74e-09
Rejected as consensus: Frequency below/above cutoff threshold.

ACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATCAACAAGTTGATT  >  NZ_CP009273/1125748‑1125920
                                                                                       |                                                                                     
aCGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTAcc                                                                                     >  2:1666418/1‑90 (MQ=255)
        aTTGTTCAGCGCCTGCCCTTCTGCGTTGGCGCAAACCCCACCCCCGCGGGCGACCACATTGCCGCGTTGCCCCGTTTTTCCCCGCCATGc                                                                             <  2:1346416/90‑1 (MQ=255)
             tCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGc                                                                        >  2:620612/1‑90 (MQ=255)
             ccAGCCCCTGCCCTTCGGGGTTGGCTCAAACCCAACCCCCCCTGGCGATCCCATTGACGAGTTCTCCCGTTTTTACCCGCCATGCCTGGc                                                                        <  1:2925583/89‑1 (MQ=255)
                  cccTGACCTTCTGCTTTGGCCCAAACCCCCCCCCCGCGGGCAATCACTTTGCCGCTTTGTCCCGTTTTTCCCCGCCATGCCTGGCGAAAc                                                                   <  2:142421/89‑1 (MQ=255)
                     tGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTgg                                                                >  1:2711043/1‑90 (MQ=255)
                     tGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTgg                                                                >  2:2869674/1‑90 (MQ=255)
                      tcccTTCTGCGTTGGCGCTAACCCCATCCCCGCGGGCGATCACATTGCCGCGTTGCCCCGCTTTTCCCCGCCATGCCTGGCGAAACTggg                                                               <  1:85089/88‑1 (MQ=255)
                      gcccTTCTGCTTTGGCGCAAACCCCATCCCCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTCCCCGCCATGCCTGGCGAAACTggg                                                               <  1:3042022/88‑1 (MQ=255)
                      gcccTTCTGCGTTGGCGCTAACCCCACCCCCGCTGGCGATCACATTGACGCGTTGTCCCGTTTTTCCCCGCCATGCCTGGCGAAACTggg                                                               <  1:2502164/88‑1 (MQ=255)
                         cTTTTGCGTTGGCGCTAACCCCACCCCCGCGGGGGATCACATTGACGCGTTGCCCCGTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTa                                                            <  2:1109171/90‑1 (MQ=255)
                         cTTCTGCGTGGGCGCAAACCCCACCCCCGCTGGCGATCACATTGACGCGTTGCCCCGTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTa                                                            <  1:2679502/90‑1 (MQ=255)
                          ttCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTaa                                                           >  1:1394338/1‑90 (MQ=255)
                          ttCTGCGTTGGCCATAACCCCCTCCCCGCGGGCGATACCATTGCCGCGTTGTCCCGTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTaa                                                           <  2:1336795/90‑1 (MQ=255)
                           tCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAAc                                                          >  1:2772975/1‑90 (MQ=255)
                           tCTGCGTGGGCGCAAACCCCACCCCCGCGGGCGATCACATTGCCGGGTTGTCCCTTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAAc                                                          <  2:1577969/90‑1 (MQ=255)
                            cTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACt                                                         >  1:1308982/1‑90 (MQ=255)
                               ctttGGCGCTAACCCCCCCACCGCGGGGAATCAAATTGGGGCTTTCCCCCTTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGa                                                      <  2:3294945/88‑1 (MQ=255)
                                gTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGAt                                                     >  2:3248467/1‑90 (MQ=255)
                                 ttGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATa                                                    >  2:2329803/1‑90 (MQ=255)
                                  tGGCGCTAACCCCATCCCCGCTGGCGATCACATTGCCGCGTTGCCCCGTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAg                                                   <  1:2890807/90‑1 (MQ=255)
                                  tGGCGCAAACCCCTTCCCCGCGGGCGATCACATTGCCGCGTTGCCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAg                                                   <  1:2152818/90‑1 (MQ=255)
                                  tGGCGCAAACCCCACCCCCGCTGGGGATCACATTGCCGGGTTGCCCCGTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAg                                                   <  2:2491727/90‑1 (MQ=255)
                                   ggCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAgg                                                  >  2:2950919/1‑90 (MQ=255)
                                         aaccccACCCCCGCGGGCGACCACTTTGCCGCTTTGTCCCGTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATc                                            <  2:2572937/87‑1 (MQ=255)
                                          cccccACCCCCGCGGGCAATCACTTTGCCGTGTTGTCCCTTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCg                                           <  2:3094630/88‑1 (MQ=255)
                                          accccATCCCCGGGGGAGATCACTTTGAGGGGTTGTCCCGCTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCg                                           <  1:1174296/88‑1 (MQ=255)
                                          accccATCCCCGCTGGCGATCAATTTCACGGGTTGTCCCGCTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCg                                           <  1:1731811/88‑1 (MQ=255)
                                          accccATCCCCGCGGGCGATCACATTGCCGGGTTGCCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCg                                           <  1:1666418/88‑1 (MQ=255)
                                          accccACCCCCGCGGGCGATCAAATTGGCGTGTTGCCCCGTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCg                                           <  1:2688939/88‑1 (MQ=255)
                                          accccACCCCCGCGGGCAATCACATTGACGCGTTGTCCCTTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCg                                           <  2:3102782/88‑1 (MQ=255)
                                          aaCCCATCCCCGCGGGGGATAATATTGGCGGGTTGTCCCGTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCg                                           <  2:3049214/90‑1 (MQ=255)
                                          aaCCCACCCCCGCGGGCGATCAAATTGACGCGTTGTCCCGTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCg                                           <  1:2641458/90‑1 (MQ=255)
                                              cATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTg                                       >  2:1284625/1‑90 (MQ=255)
                                               aTCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGa                                      >  1:60250/1‑90 (MQ=255)
                                               aTCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGa                                      >  2:2245589/1‑90 (MQ=255)
                                                   ctgCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATCGa                                  >  2:2891633/3‑90 (MQ=255)
                                                   ccGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGa                                  >  1:3305192/1‑90 (MQ=255)
                                                   ccGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGa                                  >  2:290756/1‑90 (MQ=255)
                                                   ccGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGa                                  >  1:1039452/1‑90 (MQ=255)
                                                     gCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATc                                >  2:1676424/1‑90 (MQ=255)
                                                     gCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATc                                >  2:811391/1‑90 (MQ=255)
                                                     gCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATc                                >  1:1978202/1‑90 (MQ=255)
                                                     gCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATc                                >  1:3094630/1‑90 (MQ=255)
                                                         gCGATCACTTTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCgcgc                            <  1:86911/90‑1 (MQ=255)
                                                         gCGATCACATTGACGCGTTGTCCCGTTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCgcgc                            <  1:2329803/90‑1 (MQ=255)
                                                         gCGATCACATTGACGCGTTGCCCCGTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCgcgc                            <  1:1165285/90‑1 (MQ=255)
                                                         gCGATCACATTGACGCGTTGCCCCGTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCgcgc                            <  1:1750267/90‑1 (MQ=255)
                                                         gCGATCACATGGACGCGTTGCCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCgcgc                            <  1:2527325/90‑1 (MQ=255)
                                                         gCGACCACATTGACGCGTTGCCCCGTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCgcgc                            <  1:734841/90‑1 (MQ=255)
                                                             tCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGc                        >  1:2248579/1‑90 (MQ=255)
                                                                ctttGACGCGTTGCCCCTTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTaa                     <  1:1537195/88‑1 (MQ=255)
                                                                 aTTGACGCGTTGCCCCGCTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAAt                    <  1:3142996/90‑1 (MQ=255)
                                                                       gtgTTGTCCCTTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATc              <  2:1978202/88‑1 (MQ=255)
                                                                          ttGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATcaac           >  2:1116973/1‑90 (MQ=255)
                                                                          ttGCCCCGTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATcaac           <  2:2084382/90‑1 (MQ=255)
                                                                           tgcccccCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATcaaca          <  1:320664/87‑1 (MQ=255)
                                                                           tgccccGTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATcaaca          <  1:2994001/87‑1 (MQ=255)
                                                                           tgccccGCTTTCCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATcaaca          <  1:624487/87‑1 (MQ=255)
                                                                           tGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATcaaca          >  1:3313773/1‑90 (MQ=255)
                                                                              cccGTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATCAACAAGt       <  1:807905/90‑1 (MQ=255)
                                                                              cccGTTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATCAACAAGt       <  2:417620/90‑1 (MQ=255)
                                                                              cccGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATCAACAAGt       >  1:1849211/1‑90 (MQ=255)
                                                                               ccGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATCAACAAGtt      <  1:1233724/90‑1 (MQ=255)
                                                                                cGCTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATCAACAAGttg     <  2:1207634/90‑1 (MQ=255)
                                                                                cGCTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATCAACAAGttg     <  1:2032365/90‑1 (MQ=255)
                                                                                   ttttCCCCGCCATGCCTGGCGTAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATCAACAAGttgatt  <  1:3400001/90‑1 (MQ=255)
                                                                                   ttttCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATCAACAAGttgatt  <  2:1112505/90‑1 (MQ=255)
                                                                                   ttttCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATCAACAAGttgatt  <  1:1116973/90‑1 (MQ=255)
                                                                                       |                                                                                     
ACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATCAACAAGTTGATT  >  NZ_CP009273/1125748‑1125920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: