Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,594,1610CT54.5% 46.6 / 194.6 145S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
Reads supporting (aligned to +/- strand):  ref base C (8/58);  new base T (79/0);  total (87/58)
Fisher's exact test for biased strand distribution p-value = 3.54e-32
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.31e-20
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 162 total aligned reads displayed.

GGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTA  >  NZ_CP009273/4594073‑4594250
                                                                                        |                                                                                         
ggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttct                                                                                          <  2:808142/90‑1 (MQ=255)
 gTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTtttttttt                                                                                         >  1:2633776/1‑90 (MQ=255)
   aaCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttttt                                                                                       >  2:2529419/1‑90 (MQ=255)
   aaCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttttt                                                                                       >  2:2666641/1‑90 (MQ=255)
    aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttttt                                                                                      <  1:356148/90‑1 (MQ=255)
     cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                     <  1:1248910/90‑1 (MQ=255)
     cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                     <  2:2225653/90‑1 (MQ=255)
     cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                     <  1:3012443/90‑1 (MQ=255)
         tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGcc                                                                                 >  2:1586569/1‑90 (MQ=255)
            aaGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATg                                                                              <  2:2694828/90‑1 (MQ=255)
               cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTgaga                                                                           >  2:1854588/1‑90 (MQ=255)
               cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTgaga                                                                           >  1:2689219/1‑90 (MQ=255)
               cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTgaga                                                                           >  1:2665707/1‑90 (MQ=255)
               cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCCTgaga                                                                           >  1:516059/1‑90 (MQ=255)
               cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTACTTTTTTTTCCATgaga                                                                           >  2:2942622/1‑90 (MQ=255)
                tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTTTTTTTTTTTTTTTTCCCTgagga                                                                          >  2:2476656/1‑88 (MQ=255)
                tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGaa                                                                          <  1:3384680/90‑1 (MQ=255)
                 ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGGTCTCGTTTGCGGCGGGCCTTTTTTTTTTTTTTGCCACgaggag                                                                         >  1:2977312/1‑90 (MQ=255)
                 ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTTTTTTTTTTTTTTTTTCCATGAGAAg                                                                         >  2:409233/1‑90 (MQ=255)
                 ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAg                                                                         >  1:167825/1‑90 (MQ=255)
                 ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGTCATGTGAAg                                                                         >  2:45713/1‑90 (MQ=255)
                  cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGt                                                                        <  1:404454/90‑1 (MQ=255)
                   aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATTGGAAggg                                                                       >  2:1285755/1‑88 (MQ=255)
                   aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTg                                                                       <  1:1884405/90‑1 (MQ=255)
                   aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTg                                                                       <  2:3103778/90‑1 (MQ=255)
                        tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCTTGGGAATGAgcgc                                                                  >  2:1946840/1‑90 (MQ=255)
                        tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc                                                                  <  1:1062468/90‑1 (MQ=255)
                        tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCCTGAGGAGTGAgggc                                                                  >  2:887075/1‑90 (MQ=255)
                        tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCCTGAGAAGTGAgggc                                                                  >  2:1788822/1‑90 (MQ=255)
                        tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGATTTTTTTTTTTTTTTGTCATGAGAAGGGAgcgg                                                                  >  1:1240969/1‑89 (MQ=255)
                         ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCTCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGGGCa                                                                 >  2:411323/1‑90 (MQ=255)
                         ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCa                                                                 >  1:1430385/1‑90 (MQ=255)
                         ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGGGCa                                                                 >  1:3203217/1‑90 (MQ=255)
                         ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCa                                                                 >  1:463187/1‑90 (MQ=255)
                         ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCa                                                                 <  1:2402197/90‑1 (MQ=255)
                          taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAATGATCGCag                                                                >  2:2551941/1‑90 (MQ=255)
                          taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAAGGAgcgcgg                                                                >  2:2052937/1‑88 (MQ=255)
                          taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCag                                                                <  1:1671667/90‑1 (MQ=255)
                            ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                              <  2:2561748/90‑1 (MQ=255)
                             cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCcgagg                                                             >  2:2257130/1‑89 (MQ=255)
                             cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga                                                             <  2:88220/90‑1 (MQ=255)
                             cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCagggg                                                             >  2:355113/1‑87 (MQ=255)
                              aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGTCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagagag                                                            >  1:176878/1‑90 (MQ=255)
                              aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTGCTTTTTTTTTTATTCCCTGAGAAGGGAGGGCcgagag                                                            >  1:3126044/1‑90 (MQ=255)
                              aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCagagag                                                            >  1:1928340/1‑90 (MQ=255)
                              aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGGGCagagag                                                            >  2:1196300/1‑90 (MQ=255)
                              aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCCTGAGAAGTGGGCGCagaggg                                                            >  2:2986196/1‑88 (MQ=255)
                              aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCATGAGAAGGGAGGGCagagag                                                            >  2:2884068/1‑90 (MQ=255)
                              aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCATGAGAAAGGAGGGCagaggg                                                            >  1:3217112/1‑88 (MQ=255)
                              aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGCCATTTTTTCTTTTTTTTCCCTAAGAAGGGAGAGCagagag                                                            >  1:559892/1‑90 (MQ=255)
                               gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCATGAGAAGTGAGGGCCGAGAgg                                                           >  2:2999125/1‑90 (MQ=255)
                               gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAgg                                                           >  2:1286321/1‑90 (MQ=255)
                               gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAgg                                                           >  2:365567/1‑90 (MQ=255)
                               gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAgggg                                                           >  1:1595095/1‑90 (MQ=255)
                                tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTGTCTTTTTTTTTTTTTTCCTTTAGAAGAGAGGGCAGgggggg                                                          >  2:1933917/1‑90 (MQ=255)
                                tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTGCTTTTTTCTTTTTTTTCCCTGAGAAAAGGGCGGGGAGAggg                                                          >  2:3310297/1‑90 (MQ=255)
                                tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCGGAGAggg                                                          >  2:3142711/1‑90 (MQ=255)
                                tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCCGAGAggg                                                          >  1:645466/1‑90 (MQ=255)
                                tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTGCCTGAGGAGGGGGGGCAGAGAggg                                                          >  1:2126626/1‑90 (MQ=255)
                                tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGGAGTGAGCGCAGAGAggg                                                          >  1:2315599/1‑90 (MQ=255)
                                tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATAAGAAGTGAGGGGAGAGgggg                                                          >  1:3073744/1‑90 (MQ=255)
                                tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGcc                                                                                 <  2:1530575/67‑1 (MQ=255)
                                tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGcc                                                                                 >  1:1530575/1‑67 (MQ=255)
                                tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAggg                                                          >  2:183239/1‑90 (MQ=255)
                                tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCCTTAGAAATGAGCGGagggaggg                                                          >  2:2859576/1‑90 (MQ=255)
                                tCAGGAGCCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTACATGAGAAGGGAGCGCCGAAAggg                                                          >  1:680167/1‑90 (MQ=255)
                                 cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGa                                                         <  1:1953962/90‑1 (MQ=255)
                                 cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGa                                                         <  2:406685/90‑1 (MQ=255)
                                 cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGa                                                         <  1:2261447/90‑1 (MQ=255)
                                   ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCagggagggggt                                                       >  2:2147761/1‑87 (MQ=255)
                                     aGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTAt                                                     <  2:2656513/90‑1 (MQ=255)
                                             cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctg                                             <  1:2003231/90‑1 (MQ=255)
                                               ggATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgac                                           <  1:776148/90‑1 (MQ=255)
                                                 aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAAGGAGCGCCGAGAGGGGTTATTTTGCctgactg                                         >  1:618456/1‑90 (MQ=255)
                                                 aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAGGGGTTATTTTTCctgactt                                         >  1:230402/1‑89 (MQ=255)
                                                  tGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCATGAGAAGTGAGGGCCGAGAGGGGTTATTTTTCctggctga                                        >  1:1635463/1‑90 (MQ=255)
                                                    ccATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGGGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAc                                      >  1:903197/1‑90 (MQ=255)
                                                     cATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACt                                     <  1:519637/90‑1 (MQ=255)
                                                      aTGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATGAGAAGGGAGGGCAGGGAGGGGTTATCTTGCCTGACTGAAATg                                    >  1:3229812/1‑90 (MQ=255)
                                                      aTGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTg                                    <  1:2364234/90‑1 (MQ=255)
                                                         tCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCg                                 <  2:2703752/90‑1 (MQ=255)
                                                             ggATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTg                                                                                   >  1:2236008/1‑36 (MQ=255)
                                                                tctcGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGt                          <  2:680167/90‑1 (MQ=255)
                                                                      ttGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCcgc                    <  1:1933917/90‑1 (MQ=255)
                                                                          ggcggTCATTTTTTTTTTTTTTGCCCTGAGAAGGGGGCGCAGAGGGGGGTTATTTTTCCTGACTGAACTGGCGGTTAAGTTTTCGCGGAc                >  1:345015/1‑90 (MQ=255)
                                                                            cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc              <  1:1054487/90‑1 (MQ=255)
                                                                            cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc              <  2:1194427/90‑1 (MQ=255)
                                                                            cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc              <  2:949944/90‑1 (MQ=255)
                                                                            cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc              <  2:2423743/90‑1 (MQ=255)
                                                                             ggTCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATTTTGCCTGACTGAACTGGCGGTTAAGTTTCCGCGTACCCg             >  1:1625102/1‑90 (MQ=255)
                                                                             ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCATAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCg             <  1:2856859/90‑1 (MQ=255)
                                                                             ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCg             <  2:20890/90‑1 (MQ=255)
                                                                              gTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCgg            <  1:2418646/90‑1 (MQ=255)
                                                                                 aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGt         <  1:355113/90‑1 (MQ=255)
                                                                                  ttttttCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGtt        <  1:2529419/90‑1 (MQ=255)
                                                                                   tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGttt       <  1:2052937/90‑1 (MQ=255)
                                                                                    ttttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTa      <  1:2459010/90‑1 (MQ=255)
                                                                                    ttttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTa      <  2:1311851/90‑1 (MQ=255)
                                                                                        ctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTa  >  1:1863229/1‑90 (MQ=255)
                                                                                        ctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTa  >  1:1503474/1‑90 (MQ=255)
                                                                                        |                                                                                         
GGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTA  >  NZ_CP009273/4594073‑4594250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑