| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 50,280 | T→C | F153S (TTT→TCT) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 50,280 | 0 | T | C | 98.9% | 343.2 / ‑4.2 | 90 | F153S (TTT→TCT) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (33/56); total (33/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
TTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTA > NZ_CP009273/50195‑50366 | ttCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTgag > 1:858746/1‑90 (MQ=255) ggATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTgag < 2:967045/85‑1 (MQ=255) ggATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTgag > 1:967045/1‑85 (MQ=255) ggATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCt > 1:864020/1‑90 (MQ=255) ggATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCt > 1:1686552/1‑90 (MQ=255) tACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAg < 1:2060487/90‑1 (MQ=255) atgAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAgc < 2:1301236/88‑1 (MQ=255) cGAGTCGGATGACTGGGAACCGGTATTCAGCGAATTCCACTATGCTGATGCGCAGAACTCTCACAGCTATTGCGCTGAGATTCTGGAgcg < 2:937848/90‑1 (MQ=255) cGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAgcg < 2:3153668/90‑1 (MQ=255) cGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAgcg < 2:1559085/90‑1 (MQ=255) cGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAgcg > 2:1666586/1‑90 (MQ=255) gAGCCGGATGACTGGGAATCGGTATTCCGCGAATTCCACGATGATGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAgcgg > 1:2853930/1‑90 (MQ=255) gAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAgcgg > 1:1401474/1‑90 (MQ=255) aGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAgcggc < 1:1331124/90‑1 (MQ=255) aGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAgcggc < 1:1933019/90‑1 (MQ=255) ccGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGcggcgg > 1:397649/1‑90 (MQ=255) cGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGt < 2:49384/90‑1 (MQ=255) cGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGt > 2:2511713/1‑90 (MQ=255) ggATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTa > 1:147593/1‑90 (MQ=255) gACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAAttt < 1:1816199/90‑1 (MQ=255) gACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAAttt < 2:1026802/90‑1 (MQ=255) gACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAAttt < 1:2990684/90‑1 (MQ=255) gACTGGGAACCGGTATTCAGCGAATTCCACGGTGCTGATGCGCAGAACTCTCACAGCCATTGCTTTGAGAATCTGGAGCGGCGGTAAttt < 2:3065534/90‑1 (MQ=255) gggAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGta < 2:1489776/90‑1 (MQ=255) gggAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGGAAttttttt > 2:2064392/1‑87 (MQ=255) gAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGtata < 2:1848988/90‑1 (MQ=255) gAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGtata > 1:1726155/1‑90 (MQ=255) gAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGtata < 1:2822701/90‑1 (MQ=255) gAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGGAATTTTTtata > 2:447864/1‑90 (MQ=255) aaTCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAg < 2:1530479/90‑1 (MQ=255) aTCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGa < 1:3160170/90‑1 (MQ=255) ggTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTTTTTAAAAtt > 2:2072240/1‑90 (MQ=255) ggTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAAtt > 1:3063716/1‑90 (MQ=255) aTTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTAc < 2:3046236/90‑1 (MQ=255) ttCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACg > 2:2023116/1‑90 (MQ=255) cAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGc < 1:2083330/90‑1 (MQ=255) aGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCt < 1:1185013/90‑1 (MQ=255) gCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTTTATAGAATTTACGGCTa > 1:839330/1‑90 (MQ=255) gCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTa < 1:17522/90‑1 (MQ=255) gCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTa > 1:2646442/1‑90 (MQ=255) gCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGGAATTTTTTTTTAAATTTTCGGCTa > 2:2471335/1‑90 (MQ=255) cGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAg < 2:1542583/90‑1 (MQ=255) gAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGGAATTTTGTTTAGAATTTTCGGCTAgg > 1:3074261/1‑89 (MQ=255) ttCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTTTATAAAATTTACGGCTAGCG‑cc > 1:1198844/1‑90 (MQ=255) tCCACGATGCTGATGCGCAGAACTCTCACAGCTAATGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCg < 2:3243104/90‑1 (MQ=255) aCGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGAt < 1:2023116/90‑1 (MQ=255) tGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAACG‑CCGGATGCGa > 1:1881439/1‑90 (MQ=255) gATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAAATTTGTATAAAATTTACGGCTAAGG‑CCCGAGGCGACGcc > 1:1628051/1‑90 (MQ=255) cgcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTGTAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTc < 2:2847490/90‑1 (MQ=255) cgcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTc < 2:2345050/90‑1 (MQ=255) cgcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTc < 1:891202/90‑1 (MQ=255) cgcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTc < 2:3063716/90‑1 (MQ=255) cgcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTc < 2:1899475/90‑1 (MQ=255) gcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTTTATAGAATTTACGGCTAACG‑CCGGATGCGACGCCGGGcg > 1:601993/1‑90 (MQ=255) gcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGGAATTTTTTTTAAAAATTACAGCTAACGCCCGGGTG‑GACCCCGGTcg > 2:2139268/1‑90 (MQ=255) gAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGt < 1:427318/90‑1 (MQ=255) gAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGGAATTTTTTATAGAATTTTCCGCTAGCC‑CCCGGTGCGGCGCCCGGcgcgg > 2:2641088/1‑89 (MQ=255) gAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGGAATTTTGTATAGAATTTTCGGCCAGCG‑CCGGAAGCGACACCGGTCGCGt > 1:2350767/1‑90 (MQ=255) aaCTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTc < 1:733922/90‑1 (MQ=255) acaGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCg < 1:2797773/90‑1 (MQ=255) aGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTTTATAGAATTTACGGCTAGCG‑CCGGGTGCGACCCCCGGCGCGTCTTTTCCCGc > 1:1268298/1‑90 (MQ=255) aGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGc < 1:305226/90‑1 (MQ=255) gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCTGGcc < 2:86121/90‑1 (MQ=255) gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGcc < 1:2484922/90‑1 (MQ=255) gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGcc < 1:1088959/90‑1 (MQ=255) gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGcc < 1:2713582/90‑1 (MQ=255) gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGcc < 1:26919/90‑1 (MQ=255) gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGcc < 2:3060799/90‑1 (MQ=255) gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGcc < 2:864020/90‑1 (MQ=255) gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGcc < 1:1596728/90‑1 (MQ=255) gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGcc < 2:3336082/90‑1 (MQ=255) gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGcc < 2:1054756/90‑1 (MQ=255) gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGcc < 2:799640/90‑1 (MQ=255) gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGcc < 2:591540/90‑1 (MQ=255) gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGcc < 1:2236557/90‑1 (MQ=255) gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGcc < 2:1881439/90‑1 (MQ=255) cTATTGCTCTGAGATTCTGGAGCGGCGGGAATTTTGTATTGAATTTACGGCTAACG‑GCGGGGGGGGGGCCGGTCGCGTCTTTTCCGGGCt > 2:8983/1‑90 (MQ=255) tATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGt > 2:1577901/1‑36 (MQ=38) tATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGt < 1:1577901/36‑1 (MQ=38) tATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGGCGCGTCTTTTTCCGCCtt > 1:1074407/1‑90 (MQ=255) aTTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAACG‑CCCGGTGGGGCGCCCGGCGCGTCTTTTCCGGCCTTc > 1:1362369/1‑90 (MQ=255) ttGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc < 2:642621/90‑1 (MQ=255) ttGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc < 2:1229284/90‑1 (MQ=255) ttGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc < 1:2048815/90‑1 (MQ=255) ttGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTAcc < 2:2064376/90‑1 (MQ=255) ttGCTCTGAAATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc < 1:3175833/90‑1 (MQ=255) gCTCTGAGATTCTGGAGCGGCGGTAATTTTTTATAGAATTTACAGCTAGCG‑CCGGCTGCGTCGCCGGCCTCGGCTTATTCGGCCTTcccc > 1:1570707/1‑88 (MQ=255) gCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCta < 2:2165751/90‑1 (MQ=255) gCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCta < 1:8983/90‑1 (MQ=255) gCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCta < 1:2595298/90‑1 (MQ=255) | TTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCG‑CCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTA > NZ_CP009273/50195‑50366 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |