Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,613,687	G→T	G104V (GGC→GTC)	marR →	multiple antibiotic resistance transcriptional regulator MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,613,687	0	G	T	100.0%	235.9 / NA	70	G104V (GGC→GTC)	marR	multiple antibiotic resistance transcriptional regulator MarR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (37/33);  total (37/33)

TGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTG  >  NZ_CP009273/1613600‑1613772                                                                                        |                                                                                      tGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcgtcg                                                                                     <  2:190250/90‑1 (MQ=255)  ggATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcgtcgc                                                                                    >  2:1202122/1‑90 (MQ=255)  ggATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcgtcgc                                                                                    >  2:466730/1‑90 (MQ=255)  ggATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcgtcgc                                                                                    >  2:368200/1‑90 (MQ=255)  ggATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcgtcgc                                                                                    >  2:3324039/1‑90 (MQ=255)  ggATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcgtcgc                                                                                    >  1:2286909/1‑90 (MQ=255)     tCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcgtcgcggc                                                                                 <  1:705312/90‑1 (MQ=255)      cGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCa                                                                                <  2:484422/90‑1 (MQ=255)      cGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCa                                                                                <  2:1712216/90‑1 (MQ=255)      cGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCa                                                                                <  2:2280637/90‑1 (MQ=255)      cGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCa                                                                                <  1:2608973/90‑1 (MQ=255)      cGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCa                                                                                <  2:253083/90‑1 (MQ=255)      cGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCa                                                                                <  2:256605/90‑1 (MQ=255)      cGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCa                                                                                <  2:3356191/90‑1 (MQ=255)      cGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCa                                                                                <  2:3045473/90‑1 (MQ=255)       gCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCaa                                                                               >  2:1478857/1‑90 (MQ=255)       gCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCaa                                                                               <  1:2490809/90‑1 (MQ=255)          tGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAatat                                                                            <  1:2832846/90‑1 (MQ=255)            gTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATAtgt                                                                          >  1:1534187/1‑90 (MQ=255)                gTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCa                                                                                >  2:1205728/1‑80 (MQ=255)                gTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCa                                                                                <  1:1205728/80‑1 (MQ=255)                gTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAAc                                                                      >  2:2961810/1‑90 (MQ=255)                gTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAAc                                                                      >  1:1385669/1‑90 (MQ=255)                gTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAAc                                                                      >  2:1796080/1‑90 (MQ=255)                   aaGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAAt                                                                   <  1:1156248/90‑1 (MQ=255)                   aaGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAAt                                                                   <  1:348773/90‑1 (MQ=255)                     ggCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGc                                                                 <  1:414185/90‑1 (MQ=255)                      gCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGcc                                                                >  1:2483572/1‑90 (MQ=255)                       cTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCa                                                               <  2:252253/90‑1 (MQ=255)                       cTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCa                                                               <  1:2376525/90‑1 (MQ=255)                        tGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGc                                                                 <  1:1483213/87‑1 (MQ=255)                        tGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGc                                                                 >  2:1483213/1‑87 (MQ=255)                         gggTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcgtcgc                                                                                    <  2:247804/67‑1 (MQ=255)                         gggTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcgtcgc                                                                                    >  1:247804/1‑67 (MQ=255)                          ggTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCa                                                               <  2:800303/87‑1 (MQ=255)                          ggTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCa                                                            >  2:1967440/1‑90 (MQ=255)                          ggTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCa                                                            >  1:1129930/1‑90 (MQ=255)                          ggTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCa                                                            >  1:2786948/1‑90 (MQ=255)                          ggTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGACGTCGCGGCAATATGTGAACAATGCCa                                                               >  1:800303/1‑87 (MQ=255)                               aaaGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAg                                                       >  1:2329623/1‑90 (MQ=255)                               aaaGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAg                                                       >  2:2598170/1‑90 (MQ=255)                                aaGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGt                                                      <  2:1576602/90‑1 (MQ=255)                                 aGGTTGCCGAACCCGAATGACAAGCGCGGCGTCCTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATCCCATCAATTAGtt                                                     <  1:71730/90‑1 (MQ=255)                                 aGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGtt                                                     <  1:1440692/90‑1 (MQ=255)                                    ttGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGc                                                  >  2:2074391/1‑90 (MQ=255)                                    ttGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGc                                                  >  1:3118012/1‑90 (MQ=255)                                     tGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGcc                                                 >  2:2928262/1‑90 (MQ=255)                                     tGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGcc                                                 >  1:485918/1‑90 (MQ=255)                                     tGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGcc                                                 >  2:1195256/1‑90 (MQ=255)                                      gCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCa                                                <  2:1172838/90‑1 (MQ=255)                                      gCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCa                                                <  1:1061773/90‑1 (MQ=255)                                            cccGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCt                                          <  2:1180914/90‑1 (MQ=255)                                             ccGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTg                                         <  2:2310262/90‑1 (MQ=255)                                               gAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCa                                       >  1:996852/1‑90 (MQ=255)                                                  tGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCa                                    <  2:1711206/90‑1 (MQ=255)                                                  tGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCa                                    <  1:2875730/90‑1 (MQ=255)                                                   gACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCaa                                   <  1:2598170/90‑1 (MQ=255)                                                        gcgcGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAAtt                              >  2:1213856/1‑90 (MQ=255)                                                        gcgcGGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAAtt                              >  2:1665503/1‑90 (MQ=255)                                                         cgcgGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGtt                                                     >  1:3330385/1‑66 (MQ=255)                                                         cgcgGCGTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGtt                                                     <  2:3330385/66‑1 (MQ=255)                                                          gcggcgTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCt                                          <  2:545864/76‑1 (MQ=255)                                                          gcggcgTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCt                                          >  1:545864/1‑76 (MQ=255)                                                             gcgTACTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACaa                         >  1:1821629/1‑90 (MQ=255)                                                                  cTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAc                    >  1:2496068/1‑90 (MQ=255)                                                                  cTGGTAAAACTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAAc                    >  1:1921315/1‑90 (MQ=255)                                                                       aaaaCTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAc               >  1:6911/1‑90 (MQ=255)                                                                       aaaaCTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAc               >  2:2556947/1‑90 (MQ=255)                                                                         aaCTTACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAcgg             >  1:498908/1‑90 (MQ=255)                                                                            ttACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAcggcgg          >  2:2407698/1‑90 (MQ=255)                                                                            ttACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAcggcgg          >  2:2122996/1‑90 (MQ=255)                                                                             tACCACCGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGa         <  2:498908/90‑1 (MQ=255)                                                                              accaccGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTGACAAAAAACCTGACGGCGGAc        >  2:2400261/1‑90 (MQ=255)                                                                              accaccGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGAc        >  2:2048061/1‑90 (MQ=255)                                                                              accaccGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGAc        <  1:1177317/90‑1 (MQ=255)                                                                              accaccGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGAc        >  2:2152911/1‑90 (MQ=255)                                                                              accaccGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGAc        >  2:688757/1‑90 (MQ=255)                                                                                 accGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGaa     <  1:2152911/90‑1 (MQ=255)                                                                                  ccGGCGTCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAg    >  1:3231423/1‑90 (MQ=255)                                                                                    ggcgtcGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTg  >  2:506322/1‑90 (MQ=255)                                                                                     gcgtcgcggcAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTa                             <  2:15444/62‑1 (MQ=255)                                                                                        |                                                                                      TGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTG  >  NZ_CP009273/1613600‑1613772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑