| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,340,910 | 0 | C | A | 55.6% | 5.8 / 22.6 | 18 | L30F (TTG→TTT) | pdeR | cyclic di‑GMP phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base C (6/2); new base A (0/10); total (6/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.51e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCATTTCCCTGATGCGAGCAGCCTGTTCGGCAGATAACTGCAATGTACGATCAGGTTCTGTGGTTTCGGTGGTAGAAAAGCGCAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTA > NZ_CP009273/1340827‑1340988 | gTCATTTCCCTGATGCGAGCAGCCTGTTCGGCAGATAACTGCAATGTACGATCAGGTTCTGTGGTTTCGGTGGTAGAAAAGCGCAAAACa > 2:452903/1‑90 (MQ=255) ccgcCTGTTCGGCAAATAACTGCAATGTACGATCGGGTTTTGTGGTTTGGGGGGAAAAAAAGCGAAAACCATCGCTTTTTTCCGTCAAcc < 2:580384/88‑1 (MQ=255) gcCTGTTCGGCAGATAACTGCAATGTACGATCAGGTTCTGTGGTTTCGGTGGTAGAAAAGCGCAAAACATCGCTGCTTTCCGTCAACCGc > 1:139619/1‑90 (MQ=255) ttCGGCAGATAACTGCAATGTACGATCAGGTTCTGTGGTTTCGGTGGTAGAAAAGCGAAAAACATCGCTGCTTTCCGTCAACCGCCAGTa < 1:392538/90‑1 (MQ=255) ttCGGCAGATAACTGAAATGTACGATCAGGTTCTGTGGTTTCGGTGGTAAAAAAGCGAAAAACATCGCTGCTTTCCGTCAACCGCCAGTa < 1:305078/90‑1 (MQ=255) ttCGGAAGATAACTGCAATTTACGATCAGGTTCTGTGGTTTCGGTGGTAGAAAACAGAAAAACATTGTTGCTTCCCTTCAACCGCCAGTa < 1:414292/90‑1 (MQ=255) cGGAAGATACCTGCAATGTCCGATCAGGTTTTGTGTTTTGGGTGGTAGAAAAGCGCAAAACATCGCTGCTTTCCGTCAACCGCCAGTATg < 1:452903/90‑1 (MQ=255) aaCTGCAATGTACGATCAGGTTCTGTGGTTTGGGTGGTAAAAAAGCGAAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGc < 1:242259/90‑1 (MQ=255) aaCTGAAATGTACGATCGGGTTTTGTGTTTTGGGTGGTAAAAAAGCGAAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGc < 2:49576/90‑1 (MQ=255) tCAGGTTCTGTGGTTTCGGTGGTAGAAAAGCGCAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTg > 1:293373/1‑90 (MQ=255) aGGTTCTGTGGTTTCGGTGGTAGAAAAGCGCAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTa > 1:133740/1‑90 (MQ=255) gTTCTGTGGTTTCGGTGGTAGAAAAGCGCAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACa > 2:125969/1‑90 (MQ=255) ttttgtgTTTTGGGTGGAAAAAAAGCGAAAAACATCCTTCTTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACaa < 2:133740/87‑1 (MQ=255) ttttgtgTTTTGGGTGAAAAAAAAGCCAAAACCCTCCCTGCTTTCCGCCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACaa < 2:471407/87‑1 (MQ=255) ttttgtgGTTTGGGTGGAAAAAAAGCGCAAAACACCGCTTTTTTCCGTCACCCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACaa < 2:155708/87‑1 (MQ=255) ttCTGTGTTTTCGGTGGTAAAAAAGCGAAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACaa < 1:36210/90‑1 (MQ=255) ttCTGGGTTTTGGGTGGTAAAAAAGCCAAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACaa < 1:145055/90‑1 (MQ=255) gtAGAAAAGCGCAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTa > 2:442790/1‑90 (MQ=255) | GTCATTTCCCTGATGCGAGCAGCCTGTTCGGCAGATAACTGCAATGTACGATCAGGTTCTGTGGTTTCGGTGGTAGAAAAGCGCAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTA > NZ_CP009273/1340827‑1340988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |