Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,058,9510GC54.5% 5.2 / 10.7 11A59G (GCG→GGG) yihOMFS transporter
Reads supporting (aligned to +/- strand):  ref base G (4/1);  new base C (0/6);  total (4/7)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCA  >  NZ_CP009273/4058886‑4059036
                                                                 |                                                                                     
cAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCAt                                                               >  2:29153/1‑90 (MQ=255)
       tttCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGc                                                        >  1:268400/1‑90 (MQ=255)
           cgcgAGTCGAGTAAAAACCCGGTGAGCATATCGGAAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGc                                                    <  1:209711/90‑1 (MQ=255)
            gcgAGTCGAGAAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCCCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCa                                                   <  1:154599/90‑1 (MQ=255)
                         aaaCCCGGTGAGCATATCGGTAAACGCGAAAAAAAACTTCCCCACCAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATc                                      <  1:132030/90‑1 (MQ=255)
                         aaaCCCGGTGAGAATATCGGAAAACGCGGAAAAAAACTTCCCCACCAAAAAGAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATc                                      <  1:26593/90‑1 (MQ=255)
                                       tagcGGAAACCGCGGAAAAAACCTCCCCCACCAAAAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTga                        <  1:335769/87‑1 (MQ=255)
                                            gaaaaCGCGAAAAAAACCTCCCCCACAAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGgaga                   <  2:312700/88‑1 (MQ=255)
                                              aaaCGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATa                 >  1:312700/1‑90 (MQ=255)
                                                    gaaaaaaacCTCCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGt           <  2:187045/87‑1 (MQ=255)
                                                             cTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATGCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCa  >  2:149750/1‑90 (MQ=255)
                                                                 |                                                                                     
CAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCA  >  NZ_CP009273/4058886‑4059036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑