| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,098,337 | 0 | T | G | 52.6% | 19.4 / 24.4 | 19 | G286G (GGT→GGG) | pfkA | 6‑phosphofructokinase |
| Reads supporting (aligned to +/- strand): ref base T (0/9); new base G (10/0); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGTCCGTTCAAAGGTGACTGGCTGGACTGCGC > NZ_CP009273/4098256‑4098426 | ttACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTTGGGATTCAgaa > 1:202925/1‑90 (MQ=255) tACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGGATCCAgaac > 1:214743/1‑90 (MQ=255) gTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGGCGTTGTGTAGGGATCCAGAAAGAACAGc > 2:367105/1‑90 (MQ=255) tATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCt < 1:353053/90‑1 (MQ=255) cTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGtt < 2:584246/90‑1 (MQ=255) ggCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGGCGGTTTGTTGGGATCCACAAAGAAAAGCTGGGTcc > 2:122398/1‑89 (MQ=255) cTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTATGTCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTcacc < 1:194332/90‑1 (MQ=255) tCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTcaccac < 2:477933/90‑1 (MQ=255) aTGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGGAGGGATCCAGAAAGAACAGCTGGGTCACCACGAcatc > 1:395641/1‑90 (MQ=255) ggCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACatcatc < 1:208933/90‑1 (MQ=255) gcTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGGTGTGGAGGGATCCACAACGAACAGCTGGGTCACCACCACATCATCGAc > 2:355955/1‑90 (MQ=255) tACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCt < 1:514286/90‑1 (MQ=255) tACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGGGGTTGTTTTGGGATCCACAAAGGAAACCCGGTTTACCACGACATCCTCGACGCt > 2:448944/1‑90 (MQ=255) gCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGGATCCAGAACGAACAGCTGGTTCCCCACCACATCATCGGCGCTGTc > 1:145312/1‑90 (MQ=255) tATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGa < 1:448944/90‑1 (MQ=255) tCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGaaa < 1:34938/90‑1 (MQ=255) gcggTCGTTGTGTAGGGATCCAGAAAGAACAGCTGGGTCACCCCGACATCATCGACGCTATTGAAAAAATGAAGCGGCCGGTCAAAgggg > 1:385097/1‑88 (MQ=255) gTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGTCCGTTCAAAGGTGAct < 2:214743/90‑1 (MQ=255) tCGTTGTGTAGGGATCCAGAACGAACAACTGGGTTACCCCCACATCATCGACGCTATCGAAAAAAAGAAGCGGCCGTTCAAAAGGGActg > 2:71317/1‑90 (MQ=255) tATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGTCCGTTCAAAGGTGACTGGCTGGACTgcgc < 2:395641/90‑1 (MQ=255) | TTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGTCCGTTCAAAGGTGACTGGCTGGACTGCGC > NZ_CP009273/4098256‑4098426 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |