| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,290,979 | 0 | T | G | 53.3% | 13.2 / 16.1 | 15 | T639T (ACA→ACC) | mdtO | multidrug efflux transporter permease subunit MdtO |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (8/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAAC > NZ_CP009273/4290891‑4291060 | gggCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGgtg < 2:441282/90‑1 (MQ=255) aGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCaa < 1:44847/90‑1 (MQ=255) gTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTGTTTTctat > 2:566129/1‑87 (MQ=255) gCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGgtg > 1:15835/1‑75 (MQ=255) gCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGgtg < 2:15835/75‑1 (MQ=255) gCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGGCTCTTCAAACggtt > 1:172925/1‑87 (MQ=255) cGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAACGGTAtttta > 1:448070/1‑88 (MQ=255) aCGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCg < 2:153165/90‑1 (MQ=255) tGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGcc < 2:415891/90‑1 (MQ=255) tGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAGCGTTTTTTTAGGGGAACGGCGGGGTGGGGGTGCCCGACCg > 1:509921/1‑90 (MQ=255) gTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAGCGTTTTTTGAGGAGAAAGGGTGAGGGGGGGTGGCAGACCgg > 1:1931/1‑90 (MQ=255) gTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAGCGTTATTTTGGGGGAACGGCTGGGGGGGGGTGCCAGACCgg > 1:295307/1‑90 (MQ=255) ttttAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGc < 1:514752/90‑1 (MQ=255) ttttAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGc < 2:399608/90‑1 (MQ=255) ggTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCTAGGGTTTTTTGAGGGGAACGGGTGAGGGGGGGGGGCCGACCGGGAGCGGAttt > 2:190377/1‑90 (MQ=255) ggATGGCCTGAGAGGCGGGGGTCTCTTCAAGCGGTATTTTAGGAGAAAGGGTGAGGGGGGGTGCCAGACCGGGAGCGGATTTTTCCAggg > 1:331077/1‑90 (MQ=255) tGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTTAGGGGAAAGGCTGAGTGCGGGTGCCAGAGCGGCAGCGTATTTTTCCCGGGCGt > 1:287119/1‑90 (MQ=255) gagGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAAc < 2:1931/90‑1 (MQ=255) | GGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAAC > NZ_CP009273/4290891‑4291060 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |