| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 612,012 | 0 | T | G | 54.5% | 4.7 / 11.0 | 11 | G800G (GGT→GGG) | entF | enterobactin non‑ribosomal peptide synthetase EntF |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (6/0); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCC > NZ_CP009273/611924‑612063 | gcagTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTg < 1:354894/90‑1 (MQ=255) gtgtGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGAtc < 1:136602/90‑1 (MQ=255) gATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGTTCTCTAtct > 2:493160/1‑90 (MQ=255) ggTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACt < 1:406717/90‑1 (MQ=255) aTACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGgcgc < 2:286130/90‑1 (MQ=255) cGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTTTCTTTTTCCCTCGCATTCTACTGGCGCCgg > 2:105082/1‑90 (MQ=255) ggTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTCTCTTTCTCACTGGCATTTAACTGGCGCCgggg > 1:39612/1‑89 (MQ=255) cTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGt < 2:536704/51‑1 (MQ=255) cTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTCTCTTTCTCCCTTGCATTCAACTGGGGCCgggggat > 1:62138/1‑86 (MQ=255) cTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGATCTCTTTTTCACTGGCATTCAACTGGGGCCggggggt > 1:85541/1‑86 (MQ=255) gATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGcccc < 2:85541/90‑1 (MQ=255) gATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTCTCTTTCTCACCGGCATTCAACTGGGGCCGGGGTATCTCCGGCGcccc > 1:226865/1‑90 (MQ=255) | GCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCC > NZ_CP009273/611924‑612063 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |