| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 765,675 | 0 | A | C | 53.8% | 8.5 / 12.2 | 13 | T748P (ACC→CCC) | mngB | mannosylglycerate hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (4/2); new base C (0/7); total (4/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAA > NZ_CP009273/765589‑765750 | cTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCg < 2:4795/90‑1 (MQ=255) gcCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGcc > 2:67240/1‑90 (MQ=255) tCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTcagc < 1:67240/90‑1 (MQ=255) ccGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTcagcag < 1:145038/90‑1 (MQ=255) cGTGGTCTGTTTTTTTTCCGCCTAATTTTATTGATTTATCCCGGTACCCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg < 1:306332/90‑1 (MQ=255) cGTGGTCTGTTTTCTTGTCGCCTAATTTTATTGATTTATCCCGGTCCGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg < 1:66837/90‑1 (MQ=255) cGTGGTCTGTTTTCTTGTCGCCTAATTTTATTGAGTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg < 1:21489/90‑1 (MQ=255) tgttCTGTTTTCTTGTCGCCTAAGTTTATTGATTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGAc < 1:334825/87‑1 (MQ=255) gTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTc > 1:476802/1‑90 (MQ=255) tttCTTGTCGCTTAAGTTTATTGATTTATCCCGGCCCCCCACCCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTAc < 2:454343/90‑1 (MQ=255) ttGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAgtg > 1:547456/1‑90 (MQ=255) cGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTac > 1:500009/1‑90 (MQ=255) gTTTATTGAGTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACaaaa < 1:143885/90‑1 (MQ=255) | CTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAA > NZ_CP009273/765589‑765750 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |