| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,152,727 | 0 | T | G | 53.8% | 12.0 / 15.1 | 13 | G165G (GGT→GGG) | ycfH | metal‑dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (7/0); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152652‑1152816 | aaTTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGAtct < 2:364704/90‑1 (MQ=255) aTTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGAtctc < 1:274365/90‑1 (MQ=255) tCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCgg < 1:217464/90‑1 (MQ=255) tCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATGGAgctccg > 1:338068/1‑88 (MQ=255) aagaaAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATGGATCTCGGGTTTTaaa > 1:20864/1‑88 (MQ=255) gTTTTACAGAGGACAGAGAAACGGCGGGGAAAATATTTGGTCTCGGGTTTTTAAACTCCTTTTTCTGGATTTTTGCCTTTCCTAATTCgg > 1:331536/1‑90 (MQ=255) ttACAGAGGACAGAGAAACGGCGGGGAAATTACTGGATCTCGGGTTTTAAATCTCCTTTTTCCGCAGTGGGGCCTTCCGTAATGCGGGGc > 1:349950/1‑90 (MQ=255) cAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAAc < 2:410234/90‑1 (MQ=255) ggACAGAGAAACGGCGGGGGAAATAATTGGTCTCGGGTTTTTTATTTTCTTTTTTCGCCTTTTGACCTTTCCTAATGCTGGGGAACTacg > 2:348483/1‑90 (MQ=255) ggACAGAGAAACGGCGGGGGAAATAATTGGTCTCGGGTTTTATATCTCCTTTTTTGGCGTTTTGGCCTTTCGTAATGCGGGGGGACTgcg > 2:389089/1‑90 (MQ=255) ggACAGAGAAACGGCGGGGAAATTACTGGGTCTCGGGTTTTACATCTCCTTTTCCCGCATTGTGGCCTTCCCTAATGCGGGGCAACTgcg > 1:64950/1‑90 (MQ=255) gaAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGAtgct < 1:168877/90‑1 (MQ=255) gcggGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGt < 1:211236/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 1:340108/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 1:348483/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 1:403996/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 2:172053/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 2:64950/90‑1 (MQ=255) | AATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152652‑1152816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |