| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,089,571 | 0 | A | G | 54.5% | 2.8 / 11.9 | 12 | I83V (ATT→GTT) | hisF | imidazole glycerol phosphate synthase subunit HisF |
| Reads supporting (aligned to +/- strand): ref base A (0/5); new base G (6/0); total (7/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCA > NZ_CP009273/2089484‑2089648 | gCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGAtt < 1:376970/90‑1 (MQ=255) ccAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGt < 2:196308/90‑1 (MQ=255) tGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAGTCTCTGGa > 2:40217/1‑90 (MQ=255) gtgtGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAAATg > 1:1314/1‑90 (MQ=255) cgcgTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGcgcg < 2:293082/90‑1 (MQ=255) cgTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAAATCTCTTGGAGATGCCCCGCAAAATCTTTTCTTTTGGGCggg > 2:433751/1‑89 (MQ=255) tGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGGAGATGCCCCGGAAAATCTTTTCTTTTGGGCGGGTa > 2:421690/1‑90 (MQ=255) gCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGTTTTAGGCTCTGGAAGAAGACGCGCAAAGTCTTTCTCTTGTGGCGGATaaa > 2:292713/1‑90 (MQ=255) cATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAActc < 2:358813/90‑1 (MQ=255) ttGTGTGGCGGGTGGGGTTAAGTCTCTGGAAGATGCCGCGAAAATTTTTTTCTTTTGGGCGGGTAAAATTTTCATCAACTCTCCTGCGCt > 2:102884/1‑90 (MQ=255) ttGTGTGGCGGGGGGGGGTAAGGTTCTTGAAGATGCCCCGAAAATTTTTTCCTTTTGGGGGGGGAAAAATTTCATCAACTCTCCTTCGCt > 2:310319/1‑90 (MQ=255) gtgGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCa < 1:46242/90‑1 (MQ=255) | GCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCA > NZ_CP009273/2089484‑2089648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |