Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,214,1180CA54.5% 4.4 / 13.6 11A422A (GCC→GCA) ygjGputrescine aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base A (0/6);  total (2/9)
Fisher's exact test for biased strand distribution p-value = 1.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGA  >  NZ_CP009273/3214038‑3214176
                                                                                |                                                           
gttgATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATc                                                   <  1:232982/90‑1 (MQ=255)
gttgATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATc                                                   <  2:127466/90‑1 (MQ=255)
                        ttggCCAGGGAGTTGTCCCCCCGGCGCTTACGGGTGGCGGAAACCCT‑AAAAACCCCAAAACCGACCCGCATTGACCCGCcactgacactg                           <  2:149739/87‑1 (MQ=255)
                                cGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGa                   >  2:397788/1‑90 (MQ=255)
                                 gagaTGTCCCCCCAGCGCGTCCGGGGGCCGGGAACCCAAAAAAACCCAAAAA‑CGACCCGAATTAAACCGCCACTGACACTGACCATTGaa                  <  2:294093/90‑1 (MQ=255)
                                  agttGTCCCGCCGGCGCGTACGGGGGGCCGGAACCCTCAAAACCGCAAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAAc                 <  1:296974/87‑1 (MQ=255)
                                  agaTTTCCCCCCAGCGCGTACGGGGGGCCGAAACCCTAAAAAACCCAAAAA‑CGACCCGCATTAAACCGCCACTGACACTGACCATTGAAc                 <  1:390150/90‑1 (MQ=255)
                                  agaTGTCCCCCCGGCGCGTACGGGGGGCCGAACCCCTAAAAACCCCAAAAA‑CGACCCGCATTAAACCGCCACTGACACTGACCATTGAAc                 <  2:56475/90‑1 (MQ=255)
                                              ggcgcGTTCTTGTGGCGGGAACCCAAAAAAACGAAAAAA‑CGACCCGTATTAACCCGCCACTGACACTGACCATTGAACAGTGTGAACTgg     <  1:209673/89‑1 (MQ=255)
                                                cgcgTAGTGGGGGCCGAAACGCTAAAAAACCCAAAAA‑CGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTg   <  1:408547/90‑1 (MQ=255)
                                                 gcgTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGa  >  2:70894/1‑90 (MQ=255)
                                                                               ccAAAA‑CGATCCGCATTGAACCGCcactgacactg                           <  1:100393/35‑1 (MQ=255)
                                                                               ccAAAA‑CGATCCGCATTGAACCGCcactgacactg                           >  2:100393/1‑35 (MQ=255)
                                                                                |                                                           
GTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGA  >  NZ_CP009273/3214038‑3214176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑