| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,214,126 | 0 | T | C | 53.3% | 8.7 / 16.9 | 15 | I425T (ATC→ACC) | ygjG | putrescine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base T (3/4); new base C (0/8); total (3/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.69e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGC > NZ_CP009273/3214038‑3214209 | gttgATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATc < 1:232982/90‑1 (MQ=255)gttgATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATc < 2:127466/90‑1 (MQ=255) ttggCCAGGGAGTTGTCCCCCCGGCGCTTACGGGTGGCGGAAACCCT‑AAAAACCCCAAAACCGACCCGCATTGACCCGCcactgacactg < 2:149739/87‑1 (MQ=255) cGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGa > 2:397788/1‑90 (MQ=255) gagaTGTCCCCCCAGCGCGTCCGGGGGCCGGGAACCCAAAAAAACCCAAAAA‑CGACCCGAATTAAACCGCCACTGACACTGACCATTGaa < 2:294093/90‑1 (MQ=255) agttGTCCCGCCGGCGCGTACGGGGGGCCGGAACCCTCAAAACCGCAAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 1:296974/87‑1 (MQ=255) agaTTTCCCCCCAGCGCGTACGGGGGGCCGAAACCCTAAAAAACCCAAAAA‑CGACCCGCATTAAACCGCCACTGACACTGACCATTGAAc < 1:390150/90‑1 (MQ=255) agaTGTCCCCCCGGCGCGTACGGGGGGCCGAACCCCTAAAAACCCCAAAAA‑CGACCCGCATTAAACCGCCACTGACACTGACCATTGAAc < 2:56475/90‑1 (MQ=255) ggcgcGTTCTTGTGGCGGGAACCCAAAAAAACGAAAAAA‑CGACCCGTATTAACCCGCCACTGACACTGACCATTGAACAGTGTGAACTgg < 1:209673/89‑1 (MQ=255) cgcgTAGTGGGGGCCGAAACGCTAAAAAACCCAAAAA‑CGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTg < 1:408547/90‑1 (MQ=255) gcgTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGa > 2:70894/1‑90 (MQ=255) ccAAAA‑CGATCCGCATTGAACCGCcactgacactg < 1:100393/35‑1 (MQ=255) ccAAAA‑CGATCCGCATTGAACCGCcactgacactg > 2:100393/1‑35 (MQ=255) aaa‑CGACCCGTATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGc < 1:356976/90‑1 (MQ=255) aaa‑CGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGc < 1:122560/90‑1 (MQ=255) | GTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGC > NZ_CP009273/3214038‑3214209 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |