| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,017,095 | 0 | T | G | 60.0% | 9.4 / 12.4 | 15 | V61G (GTA→GGA) | tatD | 3'‑5' ssDNA/RNA exonuclease TatD |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (9/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTACTCATCACCGGCACTAACCTGCGTGAAAGCCAGCAGGCGCAAAAGCTGGCGCGTCAGTATTCGTCCTGTTGGTCAACGGCGGGCGTACATCCTCACGACAGCAGCCAGTGGCAAGCTGCGACTGAAGAAGCGATTATTGAGCTGGCCGCGCAGCCAGAAG > NZ_CP009273/4017007‑4017169 | cTACTCATCACCGGCACTAACCTGCGTGAAAGCCAGCAGGCGCAAAAGCTGGCGCGTCAGTATTCGTCCTGTTGGTCAACGGCGGGCGTa < 2:105975/90‑1 (MQ=255) aCTCATCACCGGCACTAACCTGCGTGAAAGCCAGCAGGCGCAAAAGCTGGCGCGTCAGTATTCGTCCTGTTGGTCAACGGCGGGCGTACa < 2:182267/90‑1 (MQ=255) ggCACTAACCTGCGTGAAAGCCAGCAGGCGCAAAAGCTGGCGCGTCAGTATTCGTCCTGTTGGTCAACGGCGGGGGGACATCCCCTCGAc > 1:320440/1‑90 (MQ=255) gCGTGAAAGCCAGCAGGCGCAAAAGCTGGCGCGTCAGTATTCGTCCTGTTGGTCAACGGCGGGGGTACATCCTCCCCACAGCAGCCAggg > 1:343646/1‑88 (MQ=255) gTGAAAGCCAGCAGGCGCAAAAGCTGGCGCGTCAGTATTCGTCCTGTTGGTCAACGGCGGGCGTACATCCTCACGACAGCAGCCAGTGGc < 1:8946/90‑1 (MQ=255) aaaGCCAGCAGGCGCAAAAGCTGGCGCGTCAGTATTCGTCCTGTTGGTCAACGGCGGGGGGACATCCTCACCACCGCAGCCAGTGGCCAg > 1:355350/1‑90 (MQ=255) aGCCAGCAGGCGCAAAAGCTGGCGCGTCAGTATTCGTCCTGTTGGTCAACGGCGGGCGTACATCCTCACGACAGCAGCCAGTGGCAAGCt < 2:368391/90‑1 (MQ=255) gcgcAAAAGCTGGCGCGTCAGTATTCGTCCTGTTGGTCAACGGCGGGCGGACATCCCCCCCACAACAGCCCGGGGCAAGCCGCGACCgaa > 2:174906/1‑90 (MQ=255) ggCGCGTCAGTATTCGTCCTGTTGGTCAACGGCGGGCGGACATCCCCCCCACAGCCGCCCGGGGGAAGCGGCGGCTGAAGAAGCGagtat > 2:412792/1‑90 (MQ=255) tCCTGTTGGTCAACGGCGGGCGGACCTCCTCCCCACAACCCCCCGGGGCCAGCTGCGGCGGGAGAAGCGGGTTTTTAGCTGGgcgcgcgg > 2:233505/1‑88 (MQ=255) tCCTGTTGGTCAACGGCGGGCGGACATCCTCCCGACAGCAGCCCGTGGCAAGCTGCGGCGGAAGAAGCGGTTTTTTTGCCGGgcgcgccg > 1:305077/1‑88 (MQ=255) tGTTGGTCAACGGCGGGCGGACATCCTCCCCACAGCAGCCAGTGGCAAGCTGCGACTGGAGAAGCGAATATTTAGGTGGGCGCGCAGccc > 2:179041/1‑89 (MQ=255) tGTTGGTCAACGGCGGGCGGACATCCTCACCACAGCAGCCCGTGGGAAACTGCGACTGAAAAAGCGATTATTTAGCTGGCCGCGCCGCCa > 1:290761/1‑90 (MQ=255) tGTTGGTCAACGGCGGGCGGACATCCCCCCCACAACAGCCACTGGCAAGCTGCGGCTGAAGAAAGGAGTATTTAGGTGGCCGCgccgccc > 2:322840/1‑89 (MQ=255) ggTCAACGGCGGGCGTACATCCTCACGACAGCAGCCAGTGGCAAGCTGCGACTGAAGAAGCGATTATTGAGCTGGCCGCGCAGCCAGAAg < 2:348458/90‑1 (MQ=255) | CTACTCATCACCGGCACTAACCTGCGTGAAAGCCAGCAGGCGCAAAAGCTGGCGCGTCAGTATTCGTCCTGTTGGTCAACGGCGGGCGTACATCCTCACGACAGCAGCCAGTGGCAAGCTGCGACTGAAGAAGCGATTATTGAGCTGGCCGCGCAGCCAGAAG > NZ_CP009273/4017007‑4017169 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |