| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 50,102 | A→C | I94L (ATT→CTT) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 50,102 | 0 | A | C | 88.2% | 47.2 / ‑0.8 | 17 | I94L (ATT→CTT) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (8/7); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.81e-01 | |||||||||||
CAACCGGGTACGGACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGTGGAAGG > NZ_CP009273/50015‑50184 | cAACCGGGTACGGACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCtt > 1:269918/1‑90 (MQ=255) gACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTcgc < 2:4166/90‑1 (MQ=255) gACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTcgc > 2:226285/1‑90 (MQ=255) gACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTcgc > 2:196674/1‑90 (MQ=255) aTCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGttt > 1:129384/1‑90 (MQ=255) tAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAAc < 1:163245/90‑1 (MQ=255) gTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGtt > 2:385610/1‑90 (MQ=255) tGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTc < 1:196674/90‑1 (MQ=255) ccATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGt > 1:166231/1‑90 (MQ=255) tCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTa < 1:246676/55‑1 (MQ=255) tCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTa > 2:246676/1‑55 (MQ=255) cgcgGCGTGTGGTGACGTACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCt < 2:159760/90‑1 (MQ=255) tACCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAg < 2:324124/90‑1 (MQ=255) aCCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATc > 1:375134/1‑71 (MQ=255) aCCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATc < 2:375134/71‑1 (MQ=255) aCCAGAAATCATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGa < 1:260897/90‑1 (MQ=255) cATGGTGCTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGTGGAAgg > 1:285702/1‑90 (MQ=255) | CAACCGGGTACGGACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGTGGAAGG > NZ_CP009273/50015‑50184 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |