Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,858,499	G→C	W131C (TGG→TGC)	yeaD →	D‑hexose‑6‑phosphate mutarotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,858,499	0	G	C	100.0%	25.0 / NA	9	W131C (TGG→TGC)	yeaD	D‑hexose‑6‑phosphate mutarotase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (5/4);  total (5/4)

ACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTG  >  NZ_CP009273/1858416‑1858578                                                                                    |                                                                                aCGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc                                                                           >  1:421154/1‑90 (MQ=255) aCGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc                                                                           <  1:97143/90‑1 (MQ=255)                       aTGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCAtt                                                     >  2:153645/1‑90 (MQ=255)                        tGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCAttt                                                    >  2:259230/1‑90 (MQ=255)                             aTGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCcgcg                                               <  1:184469/90‑1 (MQ=255)                              tGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGt                                              <  1:259230/90‑1 (MQ=255)                                                      gACGCAAAGCGAAGAGACGAAAAGATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCt                      >  2:140001/1‑90 (MQ=255)                                                      gACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCt                      >  2:84675/1‑90 (MQ=255)                                                                          aaaaaTTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTg  <  1:117723/90‑1 (MQ=255)                                                                                    |                                                                                ACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTG  >  NZ_CP009273/1858416‑1858578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑