Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,236,491	A→C	F214C (TTT→TGT)	folE ←	GTP cyclohydrolase I FolE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,236,491	0	A	C	100.0%	37.7 / NA	13	F214C (TTT→TGT)	folE	GTP cyclohydrolase I FolE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (5/8);  total (5/8)

GCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGC  >  NZ_CP009273/2236406‑2236577                                                                                      |                                                                                       gCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTc                                                                                    >  2:284119/1‑90 (MQ=255)      gccgcGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGgc                                                                               >  1:297765/1‑90 (MQ=255)                          tGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTg                                                           >  2:429383/1‑90 (MQ=255)                              gTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACACACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACa                                                       <  2:18279/90‑1 (MQ=255)                                tGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAAt                                                     <  1:284119/90‑1 (MQ=255)                                      ccATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATccacca                                               >  2:154814/1‑90 (MQ=255)                                            ttCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAagagag                                         <  1:280717/90‑1 (MQ=255)                                                  ccTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGtt                                   <  1:368953/90‑1 (MQ=255)                                                       tAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGt                              <  1:344728/90‑1 (MQ=255)                                                        aaTCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTg                             <  2:413500/90‑1 (MQ=255)                                                           cAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCa                          <  1:26886/90‑1 (MQ=255)                                                                               gcgcAGACACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGcc      >  2:347371/1‑90 (MQ=255)                                                                                   aGACACTCGTGGCGCGTATTCTGTCTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCAcgc  <  1:17652/90‑1 (MQ=255)                                                                                      |                                                                                       GCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGC  >  NZ_CP009273/2236406‑2236577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑