| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,598,484 | 0 | A | G | 58.3% | 7.1 / 12.4 | 12 | I61V (ATC→GTC) | lsrD | autoinducer 2 ABC transporter permease LsrD |
| Reads supporting (aligned to +/- strand): ref base A (0/5); new base G (7/0); total (7/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGAT > NZ_CP009273/1598407‑1598572 | tGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATGtttttt > 2:297146/1‑87 (MQ=255) gCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTTTTTTGTTCGa > 2:35190/1‑90 (MQ=255) gTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGGTATTTTTTTTTGTTTGACCATCg > 2:51438/1‑90 (MQ=255) attggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTGTTTTGTTCGACCCTCGGGCTCTGCGCcctt > 2:337873/1‑90 (MQ=255) cattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCcattgcatt < 1:399811/90‑1 (MQ=255) cGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGt < 2:386333/90‑1 (MQ=255) cGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTGTTTGGTTTGACCCTCCGCCCCTGCGCCCTCGCCCCGGGGGGACTGtttt > 2:20716/1‑89 (MQ=255) aTGGTGATTGTCAGTGGCGGGGTCGATATTTTTTTTTGTTTCACCATCGGCCCCCGCGCCCTTGCATTTGGGGGTCTGTTTTTAAgtggg > 2:55930/1‑89 (MQ=255) aTGGTGATTGTCAGTGGCGGGGGCGGTATTTTGTTTTGGTTGACCCACCGCCTCTTCGCCCTTGCCTTGGGGGTACTGGTTTTAAgtggt > 2:284599/1‑90 (MQ=255) cGGGATCGATATTTCGTTTGGTTCCACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGc < 1:55930/90‑1 (MQ=255) gATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGAt < 1:127674/90‑1 (MQ=255) gATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGAt < 1:345178/90‑1 (MQ=255) | TGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGAT > NZ_CP009273/1598407‑1598572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |