Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,175,9600GT54.5% 1.3 / 11.0 11G18C (GGC→TGC) zupTzinc transporter ZupT
Reads supporting (aligned to +/- strand):  ref base G (1/4);  new base T (6/0);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTC  >  NZ_CP009273/3175887‑3176046
                                                                         |                                                                                      
gAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCCCGGTTTTTTGGGCGGTTTTCGGCGt                                                                        >  2:96264/1‑90 (MQ=255)
    aTTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTtctc                                                                    <  1:75632/90‑1 (MQ=255)
             gAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCACGGTTTTTTGTGCGTTTTTTCGCGTTTTCCGGCgaaaa                                                           >  2:377211/1‑90 (MQ=255)
                      aTGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAAc                                                  <  2:225620/90‑1 (MQ=255)
                       tGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTTTTTGCGCGTTTCTCGGCGTTTTCGGGCCAAAAACCTCGAAcc                                                 >  1:65199/1‑90 (MQ=255)
                                 tctcATTCTGACCATACTGGCGGGGGGAGCCACGGTTTTTTGGGGGTTTTTCGGCGTTTTTGGGGAAAAAACCTCGGAACGCTTTCTTGc                                       >  1:387378/1‑90 (MQ=255)
                                         tGACCATACTGGCGGGGGCAGCCACGTTTATTTGCGCGGTTTTCGGCGTTTTCCGGCAAAAAACCCCCAAACGCCTTCTGGCGGTTTTGc                               >  1:326677/1‑90 (MQ=255)
                                            ccATACTGGCGGGGGGAGCCACGTTTTTTGGCGCGTTTTTCGGCGTTCTCGGGCAAAAAACCTCGAACCGCTTTCTGGCGGTTTTGCTAg                            >  1:174410/1‑90 (MQ=255)
                                            ccATACTGGCGGGGGCAGCCACGTTTTTTTGGGCGGTTCTCGGCGGTTTCGGGCAAAAAACCTCGAACCCCTTTCTGGCGGTTTTGCttg                            >  1:323667/1‑88 (MQ=255)
                                                       ggggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg                 <  2:315897/90‑1 (MQ=255)
                                                                      aTTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGctc  <  1:274937/90‑1 (MQ=255)
                                                                         |                                                                                      
GAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTC  >  NZ_CP009273/3175887‑3176046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑